Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Apr 2

PMID 32230871

Citations 10

Authors

Denis V Voronin

Anastasiia A Kozlova

Roman A Verkhovskii

Alexey V Ermakov

Mikhail A Makarkin

Olga A Inozemtseva

Daniil N Bratashov

Affiliations

Soon will be listed here.

Abstract

Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient's life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.

Citing Articles

Validation of Artificial Intelligence (AI)-Assisted Flow Cytometry Analysis for Immunological Disorders.

Lu Z, Morita M, Yeager T, Lyu Y, Wang S, Wang Z Diagnostics (Basel). 2024; 14(4).

PMID: 38396459 PMC: 10888253. DOI: 10.3390/diagnostics14040420.

Pixel-level multimodal fusion deep networks for predicting subcellular organelle localization from label-free live-cell imaging.

Wei Z, Liu X, Yan R, Sun G, Yu W, Liu Q Front Genet. 2022; 13:1002327.

PMID: 36386823 PMC: 9644055. DOI: 10.3389/fgene.2022.1002327.

Dimensionality reduction by UMAP for visualizing and aiding in classification of imaging flow cytometry data.

Stolarek I, Samelak-Czajka A, Figlerowicz M, Jackowiak P iScience. 2022; 25(10):105142.

PMID: 36193047 PMC: 9526149. DOI: 10.1016/j.isci.2022.105142.

The Influence of Magnetic Composite Capsule Structure and Size on Their Trapping Efficiency in the Flow.

Verkhovskii R, Ermakov A, Grishin O, Makarkin M, Kozhevnikov I, Makhortov M Molecules. 2022; 27(18).

PMID: 36144805 PMC: 9501256. DOI: 10.3390/molecules27186073.

Multiple Parallel Fusion Network for Predicting Protein Subcellular Localization from Stimulated Raman Scattering (SRS) Microscopy Images in Living Cells.

Wei Z, Liu W, Yu W, Liu X, Yan R, Liu Q Int J Mol Sci. 2022; 23(18).

PMID: 36142736 PMC: 9504098. DOI: 10.3390/ijms231810827.

References

Lin S, Mao X, Huang T . Surface acoustic wave (SAW) acoustophoresis: now and beyond. Lab Chip. 2012; 12(16):2766-70. PMC: 3992433. DOI: 10.1039/c2lc90076a. View

Telford W . Near-ultraviolet laser diodes for brilliant ultraviolet fluorophore excitation. Cytometry A. 2015; 87(12):1127-37. PMC: 8335900. DOI: 10.1002/cyto.a.22686. View

Gupta A, Gupta M . Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials. 2005; 26(18):3995-4021. DOI: 10.1016/j.biomaterials.2004.10.012. View

Zhou J, Yang Y, Zhang C . Toward Biocompatible Semiconductor Quantum Dots: From Biosynthesis and Bioconjugation to Biomedical Application. Chem Rev. 2015; 115(21):11669-717. DOI: 10.1021/acs.chemrev.5b00049. View

Qu B, Wu Z, Fang F, Bai Z, Yang D, Xu S . A glass microfluidic chip for continuous blood cell sorting by a magnetic gradient without labeling. Anal Bioanal Chem. 2008; 392(7-8):1317-24. DOI: 10.1007/s00216-008-2382-4. View

Shaner N, Patterson G, Davidson M . Advances in fluorescent protein technology. J Cell Sci. 2007; 120(Pt 24):4247-60. DOI: 10.1242/jcs.005801. View

Vermesh O, Aalipour A, Ge T, Saenz Y, Guo Y, Alam I . An intravascular magnetic wire for the high-throughput retrieval of circulating tumour cells in vivo. Nat Biomed Eng. 2018; 2(9):696-705. PMC: 6261517. DOI: 10.1038/s41551-018-0257-3. View

Cristofanilli M, Budd G, Ellis M, Stopeck A, Matera J, Miller M . Circulating tumor cells, disease progression, and survival in metastatic breast cancer. N Engl J Med. 2004; 351(8):781-91. DOI: 10.1056/NEJMoa040766. View

Yang A, Soh H . Acoustophoretic sorting of viable mammalian cells in a microfluidic device. Anal Chem. 2012; 84(24):10756-62. PMC: 3677785. DOI: 10.1021/ac3026674. View

10.

Nuchtavorn N, Suntornsuk W, Lunte S, Suntornsuk L . Recent applications of microchip electrophoresis to biomedical analysis. J Pharm Biomed Anal. 2015; 113:72-96. DOI: 10.1016/j.jpba.2015.03.002. View

11.

de Bono J, Scher H, Montgomery R, Parker C, Miller M, Tissing H . Circulating tumor cells predict survival benefit from treatment in metastatic castration-resistant prostate cancer. Clin Cancer Res. 2008; 14(19):6302-9. DOI: 10.1158/1078-0432.CCR-08-0872. View

12.

DSilva J, Austin R, Sturm J . Inhibition of clot formation in deterministic lateral displacement arrays for processing large volumes of blood for rare cell capture. Lab Chip. 2015; 15(10):2240-7. PMC: 4423904. DOI: 10.1039/c4lc01409j. View

13.

Holm S, Beech J, Barrett M, Tegenfeldt J . Separation of parasites from human blood using deterministic lateral displacement. Lab Chip. 2011; 11(7):1326-32. DOI: 10.1039/c0lc00560f. View

14.

Green B, Kermanshah L, Labib M, Ahmed S, Silva P, Mahmoudian L . Isolation of Phenotypically Distinct Cancer Cells Using Nanoparticle-Mediated Sorting. ACS Appl Mater Interfaces. 2017; 9(24):20435-20443. DOI: 10.1021/acsami.7b05253. View

15.

Galanzha E, Shashkov E, Spring P, Suen J, Zharov V . In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser. Cancer Res. 2009; 69(20):7926-34. PMC: 2828368. DOI: 10.1158/0008-5472.CAN-08-4900. View

16.

Wyatt Shields 4th C, Sun D, Johnson K, Duval K, Rodriguez A, Gao L . Nucleation and growth synthesis of siloxane gels to form functional, monodisperse, and acoustically programmable particles. Angew Chem Int Ed Engl. 2014; 53(31):8070-3. DOI: 10.1002/anie.201402471. View

17.

Cushing K, Piyasena M, Carroll N, Maestas G, Lopez B, Edwards B . Elastomeric negative acoustic contrast particles for affinity capture assays. Anal Chem. 2013; 85(4):2208-15. PMC: 3621144. DOI: 10.1021/ac3029344. View

18.

Zhang G, Gurtu V, Kain S . An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem Biophys Res Commun. 1996; 227(3):707-11. DOI: 10.1006/bbrc.1996.1573. View

19.

Brown C . Fluorescence microscopy--avoiding the pitfalls. J Cell Sci. 2007; 120(Pt 10):1703-5. DOI: 10.1242/jcs.03433. View

20.

Rueden C, Schindelin J, Hiner M, DeZonia B, Walter A, Arena E . ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinformatics. 2017; 18(1):529. PMC: 5708080. DOI: 10.1186/s12859-017-1934-z. View