Efficiency of Laser-assisted Intracytoplasmic Sperm Injection in a Human Assisted Reproductive Techniques Program

Overview

Journal Clin Exp Reprod Med

Date 2012 Mar 3

PMID 22384434

Citations 5

Authors

Kyoung Hee Choi

Jung Hyun Lee

Yun Hee Yang

Tae Ki Yoon

Dong Ryul Lee

Woo Sik Lee

Affiliations

Soon will be listed here.

Abstract

Objective: Laser-assisted intracytoplasmic sperm injection (LA-ICSI), also known as micro-opening or thinning of the zona pellucida (ZP) prior to ICSI, may help to reduce mechanical damage to the oocyte during the procedure. The aim of the present study was to evaluate and analyze the efficacy of our institutional LA-ICSI program, which features laser-assisted ZP thinning prior to ICSI, in comparison with conventional ICSI (C-ICSI), performed on patients with different clinical characteristics.

Methods: Patients undergoing a total of 212 ICSI cycles were randomly divided into an LA-ICSI group (106 cycles) and a conventional ICSI group (106 cycles). To reduce tissue damage, we thinned the ZP by approximately 70%, using a laser, before ICSI. Patients thus treated formed the LA-ICSI group. Comparisons included the morphological quality of transferred embryos, blastocyst development of the remaining embryos, and clinical pregnancy, in terms of ICSI method and patient characteristics.

Results: Fertilization, development of remaining embryos, and pregnancy rate were significantly higher in the LA-ICSI group compared with the C-ICSI group. Fertilization, embryonic development, and the pregnancy rate were all improved in younger patients (<38 years of age) and in those who underwent a low number of IVF-ET attempts (<3 trials). In addition, the pregnancy rate was increased in older patients.

Conclusion: LA-ICSI may be useful in improving the chance of pregnancy in all ICSI patients.

Citing Articles

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Oocyte Degeneration After ICSI Is Not an Indicator of Live Birth in Young Women.

Hu X, Liu Y, Zhang X, Lee P, Wen Y, Ding C Front Endocrinol (Lausanne). 2021; 12:705733.

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Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage.

Ilina I, Khramova Y, Ivanova A, Filatov M, Silaeva Y, Deykin A J Assist Reprod Genet. 2020; 38(2):517-529.

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Geng T, Cheng L, Ge C, Zhang Y J Assist Reprod Genet. 2020; 37(12):2929-2945.

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