Rapid Identification of Bio-molecules Applied for Detection of Biosecurity Agents Using Rolling Circle Amplification

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Mar 3

PMID 22383994

Citations 13

Authors

Jenny Goransson

Rongqin Ke

Rachel Yuan Nong

W Mathias Howell

Anna Karman

Jan Grawe

Johan Stenberg

Malin Granberg

Magnus Elgh

David Herthnek

Per Wikstrom

Jonas Jarvius

Mats Nilsson

Affiliations

Soon will be listed here.

Abstract

Detection and identification of pathogens in environmental samples for biosecurity applications are challenging due to the strict requirements on specificity, sensitivity and time. We have developed a concept for quick, specific and sensitive pathogen identification in environmental samples. Target identification is realized by padlock- and proximity probing, and reacted probes are amplified by RCA (rolling-circle amplification). The individual RCA products are labeled by fluorescence and enumerated by an instrument, developed for sensitive and rapid digital analysis. The concept is demonstrated by identification of simili biowarfare agents for bacteria (Escherichia coli and Pantoea agglomerans) and spores (Bacillus atrophaeus) released in field.

Citing Articles

Research on safety and compliance of imported microbial inoculants using high-throughput sequencing.

Dong L, Zhang Z, Zhu B, Li S, He Y, Lou Y Front Med (Lausanne). 2022; 9:963988.

PMID: 36213630 PMC: 9532531. DOI: 10.3389/fmed.2022.963988.

Rolling Circle Amplification in Integrated Microsystems: An Uncut Gem toward Massively Multiplexed Pathogen Diagnostics and Genotyping.

Soares R, Madaboosi N, Nilsson M Acc Chem Res. 2021; 54(21):3979-3990.

PMID: 34637281 PMC: 8567418. DOI: 10.1021/acs.accounts.1c00438.

Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections.

Obande G, Singh K Infect Drug Resist. 2020; 13:455-483.

PMID: 32104017 PMC: 7024801. DOI: 10.2147/IDR.S217571.

Integration of microbead DNA handling with optomagnetic detection in rolling circle amplification assays.

Minero G, Cangiano V, Garbarino F, Fock J, Hansen M Mikrochim Acta. 2019; 186(8):528.

PMID: 31297615 DOI: 10.1007/s00604-019-3636-x.

Comparative analysis of the sensitivity of metagenomic sequencing and PCR to detect a biowarfare simulant (Bacillus atrophaeus) in soil samples.

Plaire D, Puaud S, Marsolier-Kergoat M, Elalouf J PLoS One. 2017; 12(5):e0177112.

PMID: 28472119 PMC: 5417559. DOI: 10.1371/journal.pone.0177112.

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