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Multiplexed Protein Detection by Proximity Ligation for Cancer Biomarker Validation

Overview
Journal Nat Methods
Date 2007 Mar 21
PMID 17369836
Citations 80
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Abstract

We present a proximity ligation-based multiplexed protein detection procedure in which several selected proteins can be detected via unique nucleic-acid identifiers and subsequently quantified by real-time PCR. The assay requires a 1-microl sample, has low-femtomolar sensitivity as well as five-log linear range and allows for modular multiplexing without cross-reactivity. The procedure can use a single polyclonal antibody batch for each target protein, simplifying affinity-reagent creation for new biomarker candidates.

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