Induction of ER Stress-Mediated Apoptosis by α-Lipoic Acid in A549 Cell Lines

Overview

Journal Korean J Thorac Cardiovasc Surg

Specialty Cardiology & Vascular Diseases

Date 2012 Feb 25

PMID 22363901

Citations 6

Authors

Jong In Kim

Sung-Rae Cho

Chang Min Lee

Eok-Sung Park

Ki Nyun Kim

Hyung Chul Kim

Hae Young Lee

Affiliations

Soon will be listed here.

Abstract

Background: α-Lipoic acid (α-LA) has been studied as an anticancer agent as well as a therapeutic agent for diabetes and obesity. We performed this study to evaluate the anticancer effects and mechanisms of α-LA in a lung cancer cell line, A549.

Materials And Methods: α-LA-induced apoptosis of A549 cells was detected by fluorescence-activated cell sorting analysis and a DNA fragmentation assay. Expression of apoptosis-related genes was analyzed by western blot and reverse transcription-polymerase chain reaction analyses.

Results: α-LA induced apoptosis and DNA fragmentation in A549 cells in a dose- and time-dependent manner. α-LA increased caspase activity and the degradation of poly (ADP-ribose) polymerase. It induced expression of endoplasmic reticulum (ER) stress-related genes, such as glucose-regulated protein 78, C/EBP-homologous protein, and the short form of X-box binding protein-1, and decreased expression of the anti-apoptotic protein, X-linked inhibitor of apoptosis protein. Reactive oxygen species (ROS) production was induced by α-LA, and the antioxidant N-acetyl-L-cysteine decreased the α-LA-induced increase in expression of apoptosis and ER stress-related proteins.

Conclusion: α-LA induced ER stress-mediated apoptosis in A549 cells via ROS. α-LA may therefore be clinically useful for treating lung cancer.

Citing Articles

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Biochemical and clinical relevance of alpha lipoic acid: antioxidant and anti-inflammatory activity, molecular pathways and therapeutic potential.

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Gomes M, Negrato C Diabetol Metab Syndr. 2014; 6(1):80.

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The Anti-neoplastic Effects of Alpha-Lipoic Acid: Clinical Benefits in System Tumors besides Lung Carcinomas.

Kapoor S Korean J Thorac Cardiovasc Surg. 2013; 46(2):162-3.

PMID: 23614108 PMC: 3631796. DOI: 10.5090/kjtcs.2013.46.2.162.

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