Acquired Tolerance to Oxidative Stress in Bifidobacterium Longum 105-A Via Expression of a Catalase Gene

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2012 Feb 7

PMID 22307289

Citations 11

Authors

Jianlong He

Kouta Sakaguchi

Tohru Suzuki

Affiliations

Soon will be listed here.

Abstract

For improvement of tolerance to oxidative stress in Bifidobacterium longum 105-A, we introduced the Bacillus subtilis catalase gene (katE) into it. The transformant showed catalase activity (39 U/mg crude protein) in the intracellular fraction, which increased survival by ∼100-fold after a 1-h exposure to 4.4 mM H(2)O(2), decreased de novo H(2)O(2) accumulation, and increased survival in aerated cultures by 10(5)-fold at 24 h. The protection level was better than that conferred by exogenously added catalase.

Citing Articles

Stress Response in Bifidobacteria.

Schopping M, Zeidan A, Franzen C Microbiol Mol Biol Rev. 2022; 86(4):e0017021.

PMID: 36374074 PMC: 9769877. DOI: 10.1128/mmbr.00170-21.

Recent Development of Probiotic for Treating Human Diseases.

Chen J, Chen X, Ho C Front Bioeng Biotechnol. 2022; 9:770248.

PMID: 35004640 PMC: 8727868. DOI: 10.3389/fbioe.2021.770248.

Isolation and Characterization of a Novel Cold-Active, Halotolerant Endoxylanase from sp. Nov. JL3085.

He J, Liu L, Liu X, Tang K Mar Drugs. 2020; 18(5).

PMID: 32384803 PMC: 7281462. DOI: 10.3390/md18050245.

Linoleic acid induces metabolic stress in the intestinal microorganism Bifidobacterium breve DSM 20213.

Senizza A, Rocchetti G, Callegari M, Lucini L, Morelli L Sci Rep. 2020; 10(1):5997.

PMID: 32265475 PMC: 7138814. DOI: 10.1038/s41598-020-62897-w.

Differences in microbiome and virome between cattle and horses in the same farm.

Park J, Kim E Asian-Australas J Anim Sci. 2020; 33(6):1042-1055.

PMID: 32054207 PMC: 7206377. DOI: 10.5713/ajas.19.0267.

References

Beckman J, Minor Jr R, White C, Repine J, Rosen G, Freeman B . Superoxide dismutase and catalase conjugated to polyethylene glycol increases endothelial enzyme activity and oxidant resistance. J Biol Chem. 1988; 263(14):6884-92. View

Long R, Zeng W, Chen L, Guo J, Lin Y, Huang Q . Bifidobacterium as an oral delivery carrier of oxyntomodulin for obesity therapy: inhibitory effects on food intake and body weight in overweight mice. Int J Obes (Lond). 2010; 34(4):712-9. DOI: 10.1038/ijo.2009.277. View

Chen L, Keramati L, Helmann J . Coordinate regulation of Bacillus subtilis peroxide stress genes by hydrogen peroxide and metal ions. Proc Natl Acad Sci U S A. 1995; 92(18):8190-4. PMC: 41122. DOI: 10.1073/pnas.92.18.8190. View

Mills G . Hemoglobin catabolism. I. Glutathione peroxidase, an erythrocyte enzyme which protects hemoglobin from oxidative breakdown. J Biol Chem. 1957; 229(1):189-97. View

Talwalkar A, Kailasapathy K . The role of oxygen in the viability of probiotic bacteria with reference to L. acidophilus and Bifidobacterium spp. Curr Issues Intest Microbiol. 2004; 5(1):1-8. View

Rochat T, Miyoshi A, Gratadoux J, Duwat P, Sourice S, Azevedo V . High-level resistance to oxidative stress in Lactococcus lactis conferred by Bacillus subtilis catalase KatE. Microbiology (Reading). 2005; 151(Pt 9):3011-3018. DOI: 10.1099/mic.0.27861-0. View

Guyonnet D, Chassany O, Ducrotte P, Picard C, Mouret M, Mercier C . Effect of a fermented milk containing Bifidobacterium animalis DN-173 010 on the health-related quality of life and symptoms in irritable bowel syndrome in adults in primary care: a multicentre, randomized, double-blind, controlled trial. Aliment Pharmacol Ther. 2007; 26(3):475-86. DOI: 10.1111/j.1365-2036.2007.03362.x. View

Fridovich I . Oxygen toxicity: a radical explanation. J Exp Biol. 1998; 201(Pt 8):1203-9. DOI: 10.1242/jeb.201.8.1203. View

Hamaji Y, Fujimori M, Sasaki T, Matsuhashi H, Matsui-Seki K, Shimatani-Shibata Y . Strong enhancement of recombinant cytosine deaminase activity in Bifidobacterium longum for tumor-targeting enzyme/prodrug therapy. Biosci Biotechnol Biochem. 2007; 71(4):874-83. DOI: 10.1271/bbb.60502. View

10.

Loewen P, Switala J . Multiple catalases in Bacillus subtilis. J Bacteriol. 1987; 169(8):3601-7. PMC: 212438. DOI: 10.1128/jb.169.8.3601-3607.1987. View

11.

Brioukhanov A, Netrusov A . Catalase and superoxide dismutase: distribution, properties, and physiological role in cells of strict anaerobes. Biochemistry (Mosc). 2004; 69(9):949-62. DOI: 10.1023/b:biry.0000043537.04115.d9. View

12.

Yasui K, Kano Y, Tanaka K, Watanabe K, Shimizu-Kadota M, Yoshikawa H . Improvement of bacterial transformation efficiency using plasmid artificial modification. Nucleic Acids Res. 2008; 37(1):e3. PMC: 2615632. DOI: 10.1093/nar/gkn884. View

13.

Fridovich I . Superoxide radical and superoxide dismutases. Annu Rev Biochem. 1995; 64:97-112. DOI: 10.1146/annurev.bi.64.070195.000525. View

14.

Xiao J, Kondo S, Takahashi N, Miyaji K, Oshida K, Hiramatsu A . Effects of milk products fermented by Bifidobacterium longum on blood lipids in rats and healthy adult male volunteers. J Dairy Sci. 2003; 86(7):2452-61. DOI: 10.3168/jds.S0022-0302(03)73839-9. View

15.

de Vries W, Stouthamer A . Factors determining the degree of anaerobiosis of Bifidobacterium strains. Arch Mikrobiol. 1969; 65(3):275-87. DOI: 10.1007/BF00407109. View

16.

Poupard J, Husain I, Norris R . Biology of the bifidobacteria. Bacteriol Rev. 1973; 37(2):136-65. PMC: 413809. DOI: 10.1128/br.37.2.136-165.1973. View

17.

He J, Sakaguchi K, Suzuki T . Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A. J Biosci Bioeng. 2012; 113(4):442-4. DOI: 10.1016/j.jbiosc.2011.11.019. View

18.

Kawasaki S, Mimura T, Satoh T, Takeda K, Niimura Y . Response of the microaerophilic Bifidobacterium species, B. boum and B. thermophilum, to oxygen. Appl Environ Microbiol. 2006; 72(10):6854-8. PMC: 1610298. DOI: 10.1128/AEM.01216-06. View

19.

Behl C, Davis J, Lesley R, Schubert D . Hydrogen peroxide mediates amyloid beta protein toxicity. Cell. 1994; 77(6):817-27. DOI: 10.1016/0092-8674(94)90131-7. View

20.

Gill H, Rutherfurd K, Cross M, Gopal P . Enhancement of immunity in the elderly by dietary supplementation with the probiotic Bifidobacterium lactis HN019. Am J Clin Nutr. 2001; 74(6):833-9. DOI: 10.1093/ajcn/74.6.833. View