Determination of the Ribosome-binding Sequence and Spacer Length Between Binding Site and Initiation Codon for Efficient Protein Expression in Bifidobacterium Longum 105-A

Overview

Journal J Biosci Bioeng

Specialties Biochemistry
Biomedical Engineering
Microbiology

Date 2012 Jan 6

PMID 22218059

Citations 8

Authors

Jianlong He

Kouta Sakaguchi

Tohru Suzuki

Affiliations

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Abstract

The frequency of the ribosome-binding site (RBS) and spacer length between binding site and initiation codon were analyzed using informatics, and compared the activities of various synthetic RBSs and spacers in Bifidobacterium longum 105-A. As the result, AAGGAG and a 5 nt spacer length produced the most efficient protein expression.

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