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LogSpin: a Simple, Economical and Fast Method for RNA Isolation from Infected or Healthy Plants and Other Eukaryotic Tissues

Overview
Journal BMC Res Notes
Publisher Biomed Central
Specialties Biology
General Medicine
Date 2012 Jan 21
PMID 22260178
Citations 38
Authors
Hila Yaffe
Kobi Buxdorf
Illil Shapira
Shachaf Ein-Gedi
Michal Moyal-Ben Zvi
Eyal Fridman
Menachem Moshelion
Maggie Levy
Affiliations
Soon will be listed here.
Abstract

Background: Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues or fungal mycelium.

Findings: We report on the development of a rapid and relatively inexpensive method for total RNA extraction from plants and fungus-infected tissues, as well as from insects and fungi, based on guanidine hydrochloride buffer and common DNA extraction columns originally used for the extraction and purification of plasmids and cosmids.

Conclusions: The proposed method can be used reproducibly for RNA isolation from a variety of plant species. It can also be used with infected plant tissue and fungal mycelia, which are typically recalcitrant to standard nucleic acid extraction procedures.

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