Isolation of High-quality RNA from Various Tissues of Jatropha Curcas for Downstream Applications

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 2011 Feb 9

PMID 21300020

Citations 9

Authors

G Raja Krishna Kumar

Nalini Eswaran

T Sudhakar Johnson

Affiliations

Soon will be listed here.

Abstract

A method for isolating transcriptionally active RNA for downstream applications from diverse tissues of Jatropha curcas, a plant rich in latex, lipids, waxes, polysaccharide, polyphenols, and secondary metabolites, is described. The described method uses alkaline borate buffer during tissue homogenization to negate the formation of viscous gel observed in guanidium-salt-containing methods. By this method, quality RNA was extracted from leaf, immature inflorescence, endosperm, and root tissues with yields ranging from 1.80 to 7.80mg/100mg fresh weight (FW). The total RNA obtained was found to be suitable for poly(A)(+)RNA purification, complementary DNA (cDNA) synthesis, cloning of full-length cDNA, and cDNA library construction.

Citing Articles

A Comprehensive High-Quality DNA and RNA Extraction Protocol for a Range of Cultivars and Tissue Types of the Woody Crop Avocado.

Nath O, Fletcher S, Hayward A, Shaw L, Agarwal R, Furtado A Plants (Basel). 2022; 11(3).

PMID: 35161230 PMC: 8838124. DOI: 10.3390/plants11030242.

Metatranscriptomics Reveals the Functions and Enzyme Profiles of the Microbial Community in Chinese Nong-Flavor Liquor Starter.

Huang Y, Yi Z, Jin Y, Huang M, He K, Liu D Front Microbiol. 2017; 8:1747.

PMID: 28955318 PMC: 5600954. DOI: 10.3389/fmicb.2017.01747.

Isolation of High-Quality Total RNA from Chinese Fir (Cunninghamia lanceolata (Lamb.) Hook).

Ma Z, Huang B, Xu S, Chen Y, Li S, Lin S PLoS One. 2015; 10(6):e0130234.

PMID: 26083257 PMC: 4470689. DOI: 10.1371/journal.pone.0130234.

A simple method for RNA isolation from various tissues of the tree .

Ouyang K, Li J, Huang H, Que Q, Li P, Chen X Biotechnol Biotechnol Equip. 2015; 28(6):1008-1013.

PMID: 26019587 PMC: 4434054. DOI: 10.1080/13102818.2014.981086.

Global analysis of transcriptome responses and gene expression profiles to cold stress of Jatropha curcas L.

Wang H, Zou Z, Wang S, Gong M PLoS One. 2013; 8(12):e82817.

PMID: 24349370 PMC: 3857291. DOI: 10.1371/journal.pone.0082817.