UM-SCC-104: a New Human Papillomavirus-16-positive Cancer Stem Cell-containing Head and Neck Squamous Cell Carcinoma Cell Line

Overview

Journal Head Neck

Specialties General Surgery
Oncology

Date 2011 Dec 14

PMID 22162267

Citations 60

Authors

Alice L Tang

Samantha J Hauff

John H Owen

Martin P Graham

Michael J Czerwinski

Jung Je Park

Heather Walline

Silvana Papagerakis

Jay Stoerker

Jonathan B McHugh

Douglas B Chepeha

Carol R Bradford

Thomas E Carey

Mark E Prince

Affiliations

Soon will be listed here.

Abstract

Background: Few human papillomavirus (HPV)(+) head and neck squamous cell carcinoma (HNSCC) cell lines exist. We established University of Michigan-squamous cell carcinoma-104 (UM-SCC-104), a new HPV(+) HNSCC cell line from a recurrent oral cavity tumor, and characterized it for the presence of cancer stem cells (CSCs).

Methods: Tumor cells were tested for biomarker expression by immunohistology, and the presence of HPV was assessed by several methods.

Results: UM-SCC-104 has a unique genotype, contains HPV-16, and expresses E6/E7. Inoculation of aldehyde dehydrogenase (ALDH)(+) and ALDH(-) cells in an immunocompromised mouse resulted in tumor growth from the ALDH(+) cells after 6 weeks that recapitulated the histology of the primary, whereas ALDH(-) cells did not produce tumors.

Conclusion: UM-SCC-104, a new HPV-16, CSC-containing HNSCC cell line will aid in studying recurrent HPV(+) tumors. The aggressive nature of this tumor is consistent with high uniform expression of epidermal growth factor receptor (EGFR) and a functionally significant proportion of ALDH(+) CSCs.

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