Expression of HLA-B27 in Transgenic Mice is Dependent on the Mouse H-2D Genes

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1990 Oct 1

PMID 2212952

Citations 7

Authors

C L Nickerson

J Hanson

C S David

Affiliations

Soon will be listed here.

Abstract

HLA-B27 transgenic mice in the context of various H-2 haplotypes were produced. A high expression of the HLA-B27 antigen was observed in mice homozygous for H-2b, H-2f, H-2s, H-2p, H-2r, and H-2k haplotypes. Mice of the H-2v haplotype expressed HLA-B27 at an intermediate level. Expression of HLA-B27 was minimal in mice of the H-2q and H-2d haplotypes. This was observed both on the B10 background and in DBA/2 or BALB/c mice. Only minimal expression of HLA-B27 could be detected in B10.PL (KuDd) or B10.RKDB (KkSkDdLb) mice, indicating that the low level of HLA-B27 expression maps to the H-2D gene or a very closely linked gene. Integration and transcription of the HLA-B27 gene does not appear to be different between high-expressing haplotypes and low-expressing haplotypes as determined by Southern and Northern blot analysis. However, expression of HLA-B27 on the cell surface correlated with the amount of HLA-B27 and beta 2M that could be immunoprecipitated with an anti-B27 antibody. Therefore, the association of the B27 heavy chain with endogenous beta 2M and subsequent expression on the cell surface are disrupted in mice with some class I H-2D genes. Possible mechanisms that might contribute to this defect in assembly, transport, and expression of class I molecules are discussed.

Citing Articles

No evidence for a direct role of HLA-B27 in pathological bone formation in axial SpA.

Neerinckx B, Kollnberger S, Shaw J, Lories R RMD Open. 2017; 3(1):e000451.

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Relationship between Poor Immunogenicity of HLA-A2-Restricted Peptide Epitopes and Paucity of Naïve CD8(+) T-Cell Precursors in HLA-A2-Transgenic Mice.

Choi Y, Lee D, Shin E Immune Netw. 2014; 14(4):219-25.

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Hierarchy in the assembly of HLA-B27 and HLA-Cw3 molecules in transgenic mice.

Pedrinaci S, Hanson J, David C Immunogenetics. 1994; 39(2):130-7.

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Role of human major histocompatibility complex DQ molecules in superantigenicity of streptococcus-derived protein.

Esaki Y, Fukui Y, Sudo T, Yamamoto K, Inamitsu T, Nishimura Y Infect Immun. 1994; 62(4):1228-35.

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Spontaneous inflammatory arthritis in HLA-B27 transgenic mice lacking beta 2-microglobulin: a model of human spondyloarthropathies.

Khare S, Luthra H, David C J Exp Med. 1995; 182(4):1153-8.

PMID: 7561688 PMC: 2192301. DOI: 10.1084/jem.182.4.1153.

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