Engineering Periplasmic Ligand Binding Proteins As Glucose Nanosensors

Overview

Journal Nano Rev

Specialty Biotechnology

Date 2011 Nov 24

PMID 22110874

Citations 16

Authors

Constance J Jeffery

Affiliations

Soon will be listed here.

Abstract

Diabetes affects over 100 million people worldwide. Better methods for monitoring blood glucose levels are needed for improving disease management. Several labs have previously made glucose nanosensors by modifying members of the periplasmic ligand binding protein superfamily. This minireview summarizes recent developments in constructing new versions of these proteins that are responsive within the physiological range of blood glucose levels, employ new reporter groups, and/or are more robust. These experiments are important steps in the development of novel proteins that have the characteristics needed for an implantable glucose nanosensor for diabetes management: specificity for glucose, rapid response, sensitivity within the physiological range of glucose concentrations, reproducibility, and robustness.

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