Pathogenesis and Phylogenetic Analyses of Canine Distemper Virus Strain ZJ7 Isolate from Domestic Dogs in China

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2011 Nov 18

PMID 22087872

Citations 10

Authors

Bin Tan

Yong-Jun Wen

Feng-Xue Wang

Shu-Qin Zhang

Xiu-Dong Wang

Jia-Xin Hu

Xin-Chuan Shi

Bo-Chao Yang

Li-Zhi Chen

Shi-Peng Cheng

Hua Wu

Affiliations

Soon will be listed here.

Abstract

A new isolate of canine distemper virus (CDV), named ZJ7, was isolated from lung tissues of a dog suspected with CDV infection using MDCK cells. The ZJ7 isolate induced cytopathogenic effects of syncytia in MDCK cell after six passages. In order to evaluate pathogenesis of ZJ7 strain, three CDV sero-negative dogs were intranasally inoculated with its virus suspension. All infected dogs developed clinical signs of severe bloody diarrhea, conjunctivitis, ocular discharge, nasal discharge and coughing, fever and weight loss at 21 dpi, whereas the mock group infected with DMEM were normal. The results demonstrated that CDV-ZJ7 strain isolated by MDCK cell was virulent, and the nucleotide and amino acid sequences of strain ZJ7 had no change after isolation by MDCK cell when compared with the original virus from the fresh tissues. Molecular and phylogenetic analyses for the nucleocapsid (N), phosphoprotein (P) and receptor binding haemagglutinin (H) gene of the ZJ7 isolate clearly showed it is joins to the Asia 1 group cluster of CDV strains, the predominant genotype in China.

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