Propagation of Asian Isolates of Canine Distemper Virus (CDV) in Hamster Cell Lines

Overview

Journal Acta Vet Scand

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2009 Oct 20

PMID 19835588

Citations 2

Authors

Serageldeen Sultan

Nguyen Thi Lan

Toshiki Ueda

Ryoji Yamaguchi

Ken Maeda

Kazushige Kai

Affiliations

Soon will be listed here.

Abstract

Backgrounds: The aim of this study was to confirm the propagation of various canine distemper viruses (CDV) in hamster cell lines of HmLu and BHK, since only a little is known about the possibility of propagation of CDV in rodent cells irrespective of their epidemiological importance.

Methods: The growth of CDV in hamster cell lines was monitored by titration using Vero.dogSLAMtag (Vero-DST) cells that had been proven to be susceptible to almost all field isolates of CDV, with the preparations of cell-free and cell-associated virus from the cultures infected with recent Asian isolates of CDV (13 strains) and by observing the development of cytopathic effect (CPE) in infected cultures of hamster cell lines.

Results: Eleven of 13 strains grew in HmLu cells, and 12 of 13 strains grew in BHK cells with apparent CPE of cell fusion in the late stage of infection. Two strains and a strain of Asia 1 group could not grow in HmLu cells and BHK cells, respectively.

Conclusion: The present study demonstrates at the first time that hamster cell lines can propagate the majority of Asian field isolates of CDV. The usage of two hamster cell lines suggested to be useful to characterize the field isolates biologically.

Citing Articles

First report of canine morbillivirus infection of adipose tissue-derived stem cells from dogs with distemper.

Altamirano-Samaniego F, Enciso-Benavides J, Rojas N, Manuel Iglesias-Pedraz J, Enciso N, Fossatti M Vet World. 2022; 15(7):1835-1842.

PMID: 36185532 PMC: 9394121. DOI: 10.14202/vetworld.2022.1835-1842.

Pathogenesis and phylogenetic analyses of canine distemper virus strain ZJ7 isolate from domestic dogs in China.

Tan B, Wen Y, Wang F, Zhang S, Wang X, Hu J Virol J. 2011; 8:520.

PMID: 22087872 PMC: 3229531. DOI: 10.1186/1743-422X-8-520.

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