Embryonic Stem Cell-based Mapping of Developmental Transcriptional Programs

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2011 Nov 15

PMID 22081127

Citations 46

Authors

Esteban O Mazzoni

Shaun Mahony

Michelina Iacovino

Carolyn A Morrison

George Mountoufaris

Michael Closser

Warren A Whyte

Richard A Young

Michael Kyba

David K Gifford

Hynek Wichterle

Affiliations

Soon will be listed here.

Abstract

The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.

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