Tumor Necrosis Factor-like Weak Inducer of Apoptosis (TWEAK) Mediates P38 Mitogen-activated Protein Kinase Activation and Signal Transduction in Peripheral Blood Mononuclear Cells from Patients with Lupus Nephritis

Overview

Journal Inflammation

Specialties Allergy & Immunology
Pathology

Date 2011 Oct 20

PMID 22009442

Citations 15

Authors

Liu Zhi-Chun

Zhou Qiao-Ling

Liu Zhi-Qin

Li Xiao-Zhao

Zuo Xiao-xia

Tang Rong

Affiliations

Soon will be listed here.

Abstract

Forty-two patients with systemic lupus erythematosus (SLE), including 26 patients with renal damage and 16 without, and 20 healthy controls were included in the study. The isolated peripheral blood mononuclear cells (PBMCs) were treated with a p38 inhibitor (SB203580) or anti-tumor necrosis factor-like weak inducer of apoptosis (TWEAK) mAb, with or without phytohemagglutinin/phorbol myristate acetate (PHA/PMA) stimulation. Western blot experiments were used to evaluate the protein expression of TWEAK and p38 MAPK in PBMCs .Next, the contents of interleukin-10 (IL-10) and monocyte chemoattractant protein-1 (MCP-1) in the supernatant were measured by ELISA. The results showed that expression of TWEAK protein in PBMCs from lupus nephritis patients was significantly higher than that from SLE patients without renal damage and healthy controls. PHA/PMA simulation could upregulate the productions of TWEAK and p-p38MAPK in PBMCs from patients with SLE. Anti-TWEAK mAb treatment downregulated both TWEAK and p-p38 MAPK expression in PBMCs, as well as IL-10 and MCP-1 in the supernatant; SB203580 had the same effect on cytokine production in PBMC, but had no effect on the expression of TWEAK. Our results suggested that TWEAK-p38 MAPK-IL-10, MCP-1 signaling pathway in PBMC played an important pathogenic role in lupus nephritis.

Citing Articles

Diagnostic value of TWEAK for predicting active lupus nephritis in patients with systemic lupus erythematosus: a systematic review and meta-analysis.

Ma H, Chen S, Cao W, Min C Ren Fail. 2020; 43(1):20-31.

PMID: 33307926 PMC: 7745842. DOI: 10.1080/0886022X.2020.1853568.

Association of S100B polymorphisms and serum S100B with risk of systemic lupus erythematous in a Chinese population.

Lu Y, Huang H, Liu C, Zeng Y, Wang R, Wang C Genet Mol Biol. 2019; 42(2):321-328.

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Association of Platelet Binding to Lymphocytes with B Cell Abnormalities and Clinical Manifestations in Systemic Lupus Erythematosus.

Zamora C, Toniolo E, Diaz-Torne C, Canto E, Magallares B, Ortiz M Mediators Inflamm. 2019; 2019:2473164.

PMID: 30944545 PMC: 6421767. DOI: 10.1155/2019/2473164.

Tumor Necrosis Factor-Like Weak Inducer of Apoptosis Activates Type I Interferon Signals in Lupus Nephritis.

Xue L, Liu L, Huang J, Wen J, Yang R, Bo L Biomed Res Int. 2018; 2017:4927376.

PMID: 29333443 PMC: 5733219. DOI: 10.1155/2017/4927376.

TWEAK protects cardiomyocyte against apoptosis in a PI3K/AKT pathway dependent manner.

Yang B, Yan P, Gong H, Zuo L, Shi Y, Guo J Am J Transl Res. 2016; 8(9):3848-3860.

PMID: 27725864 PMC: 5040682.

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