Dysregulation of Gene Expression in the Artificial Human Trisomy Cells of Chromosome 8 Associated with Transformed Cell Phenotypes

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2011 Oct 8

PMID 21980425

Citations 27

Authors

Hisakatsu Nawata

Genro Kashino

Keizo Tano

Kazuhiro Daino

Yoshiya Shimada

Hiroyuki Kugoh

Mitsuo Oshimura

Masami Watanabe

Affiliations

Soon will be listed here.

Abstract

A change in chromosome number, known as aneuploidy, is a common characteristic of cancer. Aneuploidy disrupts gene expression in human cancer cells and immortalized human epithelial cells, but not in normal human cells. However, the relationship between aneuploidy and cancer remains unclear. To study the effects of aneuploidy in normal human cells, we generated artificial cells of human primary fibroblast having three chromosome 8 (trisomy 8 cells) by using microcell-mediated chromosome transfer technique. In addition to decreased proliferation, the trisomy 8 cells lost contact inhibition and reproliferated after exhibiting senescence-like characteristics that are typical of transformed cells. Furthermore, the trisomy 8 cells exhibited chromosome instability, and the overall gene expression profile based on microarray analyses was significantly different from that of diploid human primary fibroblasts. Our data suggest that aneuploidy, even a single chromosome gain, can be introduced into normal human cells and causes, in some cases, a partial cancer phenotype due to a disruption in overall gene expression.

Citing Articles

Patterns of Aneuploidy and Signaling Consequences in Cancer.

Zhakula-Kostadinova N, Taylor A Cancer Res. 2024; 84(16):2575-2587.

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Characteristics and expression of lncRNA and transposable elements in aneuploidy.

Zhang S, Wang R, Zhu X, Zhang L, Liu X, Sun L iScience. 2023; 26(12):108494.

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Trisomies Reorganize Human 3D Genome.

Zhegalova I, Vasiluev P, Flyamer I, Shtompel A, Glazyrina E, Shilova N Int J Mol Sci. 2023; 24(22).

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Modeling specific aneuploidies: from karyotype manipulations to biological insights.

Truong M, Cane-Gasull P, Lens S Chromosome Res. 2023; 31(3):25.

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Regulation of protein complex partners as a compensatory mechanism in aneuploid tumors.

Senger G, Santaguida S, Schaefer M Elife. 2022; 11.

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