» Articles » PMID: 8266105

WT1-mediated Growth Suppression of Wilms Tumor Cells Expressing a WT1 Splicing Variant

Overview
Journal Science
Specialty Science
Date 1993 Dec 24
PMID 8266105
Citations 37
Authors
Affiliations
Soon will be listed here.
Abstract

A human Wilms tumor cell line (RM1) was developed to test the tumor suppressor activity of WT1, a zinc finger transcription factor that is expressed in the developing human kidney and is mutationally inactivated in a subset of Wilms tumors. Transfection of each of four wild-type WT1 isoforms suppressed the growth of RM1 cells. The endogenous WT1 transcript in these cells was devoid of exon 2 sequences, a splicing alteration that was also detected in varying amounts in all Wilms tumors tested but not in normal kidney. Production of this abnormal transcript, which encodes a functionally altered protein, may represent a distinct mechanism for inactivating WT1 in Wilms tumors.

Citing Articles

RNA-Binding Proteins in Acute Leukemias.

Schuschel K, Helwig M, Huttelmaier S, Heckl D, Klusmann J, Hoell J Int J Mol Sci. 2020; 21(10).

PMID: 32408494 PMC: 7279408. DOI: 10.3390/ijms21103409.


Antitumor activity of CAR-T cells targeting the intracellular oncoprotein WT1 can be enhanced by vaccination.

Akahori Y, Wang L, Yoneyama M, Seo N, Okumura S, Miyahara Y Blood. 2018; 132(11):1134-1145.

PMID: 30045840 PMC: 6148344. DOI: 10.1182/blood-2017-08-802926.


Therapeutic Potency of Nanoformulations of siRNAs and shRNAs in Animal Models of Cancers.

Karim M, Tha K, Othman I, Uddin M, Chowdhury E Pharmaceutics. 2018; 10(2).

PMID: 29861465 PMC: 6026921. DOI: 10.3390/pharmaceutics10020065.


WT1 Alternative Splicing: Role of Its Isoforms in Neuroblastoma.

Rasa D, DAmico A, Maugeri G, Cavallaro S, DAgata V J Mol Neurosci. 2017; 62(2):131-141.

PMID: 28534317 DOI: 10.1007/s12031-017-0930-0.


Expression profile of Wilms Tumor 1 (WT1) isoforms in undifferentiated and all-trans retinoic acid differentiated neuroblastoma cells.

Maugeri G, DAmico A, Rasa D, Reitano R, Saccone S, Federico C Genes Cancer. 2016; 7(1-2):47-58.

PMID: 27014421 PMC: 4773705. DOI: 10.18632/genesandcancer.94.