Enhanced Sensitivity of Lateral Flow Tests Using a Two-dimensional Paper Network Format

Overview

Journal Anal Chem

Specialty Chemistry

Date 2011 Sep 23

PMID 21936486

Citations 61

Authors

Elain Fu

Tinny Liang

Jared Houghtaling

Sujatha Ramachandran

Stephen A Ramsey

Barry Lutz

Paul Yager

Affiliations

Soon will be listed here.

Abstract

Point-of-care diagnostic assays that are rapid, easy-to-use, and low-cost are needed for use in low-resource settings; the lateral flow test has become the standard bioassay format in such settings because it meets those criteria. However, for a number of analytes, conventional lateral flow tests lack the sensitivity needed to have clinical utility. To address this limitation, we are developing a paper network platform that extends the conventional lateral flow test to two dimensions. The two-dimensional structures allow incorporation of multistep processes for improved sensitivity, while still retaining the positive aspects of conventional lateral flow tests. Here we create an easy-to-use, signal-amplified immunoassay based on a modified commercial strip test for human chorionic gonadotropin, the hormone used to detect pregnancy, and demonstrate an improved limit of detection compared to a conventional lateral flow assay. These results highlight the potential of the paper network platform to enhance access to high-quality diagnostic capabilities in low-resource settings in the developed and developing worlds.

Citing Articles

Transitions in Immunoassay Leading to Next-Generation Lateral Flow Assays and Future Prospects.

Fujiuchi K, Aoki N, Ohtake T, Iwashita T, Kawasaki H Biomedicines. 2024; 12(10).

PMID: 39457581 PMC: 11504701. DOI: 10.3390/biomedicines12102268.

Sensitive Colorimetric Lateral Flow Assays Enabled by Platinum-Group Metal Nanoparticles with Peroxidase-Like Activities.

Shao S, Wang X, Sorial C, Sun X, Xia X Adv Healthc Mater. 2024; :e2401677.

PMID: 39108051 PMC: 11799360. DOI: 10.1002/adhm.202401677.

The Evaluation of a Lateral Flow Strip Based on the Covalently Fixed "End-On" Orientation of an Antibody for Detection.

Thongmee P, Ngernpimai S, Srichaiyapol O, Mongmonsin U, Teerasong S, Charoensri N Anal Chem. 2024; 96(21):8543-8551.

PMID: 38748432 PMC: 11140673. DOI: 10.1021/acs.analchem.4c00533.

Dual sensitivity enhancement in gold nanoparticle-based lateral flow immunoassay for visual detection of carcinoembryonic antigen.

Mahmoudi T, Shirdel B, Mansoori B, Baradaran B Anal Sci Adv. 2024; 1(3):161-172.

PMID: 38716129 PMC: 10989072. DOI: 10.1002/ansa.202000023.

Post-Assay Chemical Enhancement for Highly Sensitive Lateral Flow Immunoassays: A Critical Review.

Panferov V, Zherdev A, Dzantiev B Biosensors (Basel). 2023; 13(9).

PMID: 37754100 PMC: 10526817. DOI: 10.3390/bios13090866.

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