Annexin A2 Binds RNA and Reduces the Frameshifting Efficiency of Infectious Bronchitis Virus

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2011 Sep 16

PMID 21918681

Citations 23

Authors

Hoyun Kwak

Min Woo Park

Sunjoo Jeong

Affiliations

Soon will be listed here.

Abstract

Annexin A2 (ANXA2) is a protein implicated in diverse cellular functions, including exocytosis, DNA synthesis and cell proliferation. It was recently proposed to be involved in RNA metabolism because it was shown to associate with some cellular mRNA. Here, we identified ANXA2 as a RNA binding protein (RBP) that binds IBV (Infectious Bronchitis Virus) pseudoknot RNA. We first confirmed the binding of ANXA2 to IBV pseudoknot RNA by ultraviolet crosslinking and showed its binding to RNA pseudoknot with ANXA2 protein in vitro and in the cells. Since the RNA pseudoknot located in the frameshifting region of IBV was used as bait for cellular RBPs, we tested whether ANXA2 could regulate the frameshfting of IBV pseudoknot RNA by dual luciferase assay. Overexpression of ANXA2 significantly reduced the frameshifting efficiency from IBV pseudoknot RNA and knockdown of the protein strikingly increased the frameshifting efficiency. The results suggest that ANXA2 is a cellular RBP that can modulate the frameshifting efficiency of viral RNA, enabling it to act as an anti-viral cellular protein, and hinting at roles in RNA metabolism for other cellular mRNAs.

Citing Articles

Biological Significance and Therapeutic Promise of Programmed Ribosomal Frameshifting.

Ramamonjiharisoa M, Liu S Int J Mol Sci. 2025; 26(3).

PMID: 39941062 PMC: 11818727. DOI: 10.3390/ijms26031294.

Annexin A2: the missing piece in the puzzle of pathogen-induced damage.

Li C, Yu J, Liao D, Su X, Yi X, Yang X Virulence. 2023; 14(1):2237222.

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RNA-binding is an ancient trait of the Annexin family.

Patil S, Panchal V, Rostbo T, Romanyuk S, Hollas H, Brenk R Front Cell Dev Biol. 2023; 11:1161588.

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Thinking Outside the Frame: Impacting Genomes Capacity by Programmed Ribosomal Frameshifting.

Riegger R, Caliskan N Front Mol Biosci. 2022; 9:842261.

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The Annexin A2/S100A10 Complex: The Mutualistic Symbiosis of Two Distinct Proteins.

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