Persistence of Environmental DNA in Freshwater Ecosystems

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2011 Aug 23

PMID 21858099

Citations 125

Authors

Tony Dejean

Alice Valentini

Antoine Duparc

Stephanie Pellier-Cuit

Francois Pompanon

Pierre Taberlet

Claude Miaud

Affiliations

Soon will be listed here.

Abstract

The precise knowledge of species distribution is a key step in conservation biology. However, species detection can be extremely difficult in many environments, specific life stages and in populations at very low density. The aim of this study was to improve the knowledge on DNA persistence in water in order to confirm the presence of the focus species in freshwater ecosystems. Aquatic vertebrates (fish: Siberian sturgeon and amphibian: Bullfrog tadpoles) were used as target species. In control conditions (tanks) and in the field (ponds), the DNA detectability decreases with time after the removal of the species source of DNA. DNA was detectable for less than one month in both conditions. The density of individuals also influences the dynamics of DNA detectability in water samples. The dynamics of detectability reflects the persistence of DNA fragments in freshwater ecosystems. The short time persistence of detectable amounts of DNA opens perspectives in conservation biology, by allowing access to the presence or absence of species e.g. rare, secretive, potentially invasive, or at low density. This knowledge of DNA persistence will greatly influence planning of biodiversity inventories and biosecurity surveys.

Citing Articles

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Is it worth the extra mile? Comparing environmental DNA and RNA metabarcoding for vertebrate and invertebrate biodiversity surveys in a lowland stream.

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eDNA-Based Survey of Fish Species in Water Bodies Using Loop-Mediated Isothermal Amplification (LAMP) for Application of Developing Automatic Sampler.

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