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A Unique BSL-3 Cryo-Electron Microscopy Laboratory at UTMB

Overview
Journal Appl Biosaf
Publisher Mary Ann Liebert
Date 2011 Aug 20
PMID 21852942
Citations 3
Authors
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Abstract

This article describes a unique cryo-electron microscopy (CryoEM) facility to study the three-dimensional organization of viruses at biological safety level 3 (BSL-3). This facility, the W. M. Keck Center for Virus Imaging, has successfully operated for more than a year without incident and was cleared for select agent studies by the Centers for Disease Control and Prevention (CDC). Standard operating procedures for the laboratory were developed and implemented to ensure its safe and efficient operation. This facility at the University of Texas Medical Branch (Galveston, TX) is the only such BSL-3 CryoEM facility approved for select agent research.

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References
1.
Neuman B, Adair B, Yoshioka C, Quispe J, Orca G, Kuhn P . Supramolecular architecture of severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy. J Virol. 2006; 80(16):7918-28. PMC: 1563832. DOI: 10.1128/JVI.00645-06. View

2.
Freiberg A, Sherman M, Morais M, Holbrook M, Watowich S . Three-dimensional organization of Rift Valley fever virus revealed by cryoelectron tomography. J Virol. 2008; 82(21):10341-8. PMC: 2573222. DOI: 10.1128/JVI.01191-08. View

3.
Sherman M, Freiberg A, Holbrook M, Watowich S . Single-particle cryo-electron microscopy of Rift Valley fever virus. Virology. 2009; 387(1):11-5. PMC: 2673237. DOI: 10.1016/j.virol.2009.02.038. View