Single Cell Transcriptomics of Neighboring Hyphae of Aspergillus Niger

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2011 Aug 6

PMID 21816052

Citations 36

Authors

Charissa de Bekker

Oskar Bruning

Martijs J Jonker

Timo M Breit

Han A B Wosten

Affiliations

Soon will be listed here.

Abstract

Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories.

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