Chestnut Extract Induces Apoptosis in AGS Human Gastric Cancer Cells

Overview

Journal Nutr Res Pract

Specialty Nutritional Sciences

Date 2011 Jul 23

PMID 21779520

Citations 4

Authors

Hyun Sook Lee

Eun Ji Kim

Sun Hyo Kim

Affiliations

Soon will be listed here.

Abstract

In Korea, chestnut production is increasing each year, but consumption is far below production. We investigated the effect of chestnut extracts on antioxidant activity and anticancer effects. Ethanol extracts of raw chestnut (RCE) or chestnut powder (CPE) had dose-dependent superoxide scavenging activity. Viable numbers of MDA-MD-231 human breast cancer cells, DU145 human prostate cancer cells, and AGS human gastric cancer cells decreased by 18, 31, and 69%, respectively, following treatment with 200 µg/mL CPE for 24 hr. CPE at various concentrations (0-200 µg/mL) markedly decreased AGS cell viability and increased apoptotic cell death dose and time dependently. CPE increased the levels of cleaved caspase-8, -7, -3, and poly (ADP-ribose) polymerase in a dose-dependent manner but not cleaved caspase-9. CPE exerted no effects on Bcl-2 and Bax levels. The level of X-linked inhibitor of apoptosis protein decreased within a narrow range following CPE treatment. The levels of Trail, DR4, and Fas-L increased dose-dependently in CPE-treated AGS cells. These results show that CPE decreases growth and induces apoptosis in AGS gastric cancer cells and that activation of the death receptor pathway contributes to CPE-induced apoptosis in AGS cells. In conclusion, CPE had more of an effect on gastric cancer cells than breast or prostate cancer cells, suggesting that chestnuts would have a positive effect against gastric cancer.

Citing Articles

Benzyl Isothiocyanate Induces Apoptosis via Reactive Oxygen Species-Initiated Mitochondrial Dysfunction and DR4 and DR5 Death Receptor Activation in Gastric Adenocarcinoma Cells.

Han K, Po W, Sohn U, Kim H Biomolecules. 2019; 9(12).

PMID: 31817791 PMC: 6995572. DOI: 10.3390/biom9120839.

Suppression of Nrf2 Activity by Chestnut Leaf Extract Increases Chemosensitivity of Breast Cancer Stem Cells to Paclitaxel.

Woo Y, Oh J, Kim J Nutrients. 2017; 9(7).

PMID: 28718813 PMC: 5537874. DOI: 10.3390/nu9070760.

Potential Anticancer Effects of Polyphenols from Chestnut Shell Extracts: Modulation of Cell Growth, and Cytokinomic and Metabolomic Profiles.

Sorice A, Siano F, Capone F, Guerriero E, Picariello G, Budillon A Molecules. 2016; 21(10).

PMID: 27775667 PMC: 6273950. DOI: 10.3390/molecules21101411.

Radish (Raphanus sativus L. leaf) ethanol extract inhibits protein and mRNA expression of ErbB(2) and ErbB(3) in MDA-MB-231 human breast cancer cells.

Kim W, Kim J, Jeong D, Chun Y, Kim S, Cho K Nutr Res Pract. 2011; 5(4):288-93.

PMID: 21994522 PMC: 3180678. DOI: 10.4162/nrp.2011.5.4.288.

References

Tong Q, Zheng L, Wang L, Zeng F, Chen F, Dong J . Downregulation of XIAP expression induces apoptosis and enhances chemotherapeutic sensitivity in human gastric cancer cells. Cancer Gene Ther. 2005; 12(5):509-14. DOI: 10.1038/sj.cgt.7700813. View

Lorenzo H, Susin S . Therapeutic potential of AIF-mediated caspase-independent programmed cell death. Drug Resist Updat. 2008; 10(6):235-55. DOI: 10.1016/j.drup.2007.11.001. View

Budihardjo I, Oliver H, Lutter M, Luo X, Wang X . Biochemical pathways of caspase activation during apoptosis. Annu Rev Cell Dev Biol. 1999; 15:269-90. DOI: 10.1146/annurev.cellbio.15.1.269. View

Baker S, Reddy E . Modulation of life and death by the TNF receptor superfamily. Oncogene. 1999; 17(25):3261-70. DOI: 10.1038/sj.onc.1202568. View

Oliver L, Vallette F . The role of caspases in cell death and differentiation. Drug Resist Updat. 2005; 8(3):163-70. DOI: 10.1016/j.drup.2005.05.001. View

LaCasse E, Cherton-Horvat G, Hewitt K, Jerome L, Morris S, Kandimalla E . Preclinical characterization of AEG35156/GEM 640, a second-generation antisense oligonucleotide targeting X-linked inhibitor of apoptosis. Clin Cancer Res. 2006; 12(17):5231-41. DOI: 10.1158/1078-0432.CCR-06-0608. View

Strater J, Moller P . CD95 (Fas/APO-1)/CD95L in the gastrointestinal tract: fictions and facts. Virchows Arch. 2003; 442(3):218-25. DOI: 10.1007/s00428-003-0760-z. View

Chen C, Kong A . Dietary cancer-chemopreventive compounds: from signaling and gene expression to pharmacological effects. Trends Pharmacol Sci. 2005; 26(6):318-26. DOI: 10.1016/j.tips.2005.04.004. View

Korkolopoulou P, Saetta A, Levidou G, Gigelou F, Lazaris A, Thymara I . c-FLIP expression in colorectal carcinomas: association with Fas/FasL expression and prognostic implications. Histopathology. 2007; 51(2):150-6. DOI: 10.1111/j.1365-2559.2007.02723.x. View

10.

Mahalingam D, Szegezdi E, Keane M, de Jong S, Samali A . TRAIL receptor signalling and modulation: Are we on the right TRAIL?. Cancer Treat Rev. 2009; 35(3):280-8. DOI: 10.1016/j.ctrv.2008.11.006. View

11.

Qiao L, Wong B . Targeting apoptosis as an approach for gastrointestinal cancer therapy. Drug Resist Updat. 2009; 12(3):55-64. DOI: 10.1016/j.drup.2009.02.002. View

12.

Cho H, Kim W, Kim E, Jung K, Park S, Lee H . Conjugated linoleic acid inhibits cell proliferation and ErbB3 signaling in HT-29 human colon cell line. Am J Physiol Gastrointest Liver Physiol. 2003; 284(6):G996-1005. DOI: 10.1152/ajpgi.00347.2002. View

13.

Lim S . Fas-related apoptosis in gastric adenocarcinoma. Oncol Rep. 2002; 10(1):57-63. View

14.

Thorburn A, Behbakht K, Ford H . TRAIL receptor-targeted therapeutics: resistance mechanisms and strategies to avoid them. Drug Resist Updat. 2008; 11(1-2):17-24. PMC: 2435079. DOI: 10.1016/j.drup.2008.02.001. View

15.

Na M, Seo E, Kim W . Effects of alpha-lipoic acid on cell proliferation and apoptosis in MDA-MB-231 human breast cells. Nutr Res Pract. 2010; 3(4):265-71. PMC: 2809232. DOI: 10.4162/nrp.2009.3.4.265. View

16.

Verhagen A, Silke J, Ekert P, Pakusch M, Kaufmann H, Connolly L . HtrA2 promotes cell death through its serine protease activity and its ability to antagonize inhibitor of apoptosis proteins. J Biol Chem. 2001; 277(1):445-54. DOI: 10.1074/jbc.M109891200. View

17.

Ashkenazi A, Dixit V . Death receptors: signaling and modulation. Science. 1998; 281(5381):1305-8. DOI: 10.1126/science.281.5381.1305. View

18.

Kim E, Kang I, Cho H, Kim W, Ha Y, Yoon Park J . Conjugated linoleic acid downregulates insulin-like growth factor-I receptor levels in HT-29 human colon cancer cells. J Nutr. 2003; 133(8):2675-81. DOI: 10.1093/jn/133.8.2675. View

19.

Nagashima H, Mori M, Sadanaga N, Mashino K, Yoshikawa Y, Sugimachi K . Expression of Fas ligand in gastric carcinoma relates to lymph node metastasis. Int J Oncol. 2001; 18(6):1157-62. DOI: 10.3892/ijo.18.6.1157. View

20.

Vekiari S, Gordon M, Garcia-Macias P, Labrinea H . Extraction and determination of ellagic acid contentin chestnut bark and fruit. Food Chem. 2015; 110(4):1007-11. DOI: 10.1016/j.foodchem.2008.02.005. View