Oxygen-dependent Delayed Fluorescence Measured in Skin After Topical Application of 5-aminolevulinic Acid

Overview

Journal J Biophotonics

Date 2011 Jul 20

PMID 21770036

Citations 12

Authors

Floor A Harms

Wadim M I de Boon

Gianmarco M Balestra

Sander I A Bodmer

Tanja Johannes

Robert J Stolker

Egbert G Mik

Affiliations

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Abstract

Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that delayed fluorescence is readily observed from skin in rat and man after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Delayed fluorescence lifetimes respond to changes in inspired oxygen fraction and blood supply. The signals contain lifetime distributions and the fitting of rectangular distributions to the data appears more adequate than mono-exponential fitting. The use of topically applied ALA for delayed fluorescence lifetime measurements might pave the way for clinical use of this technique.

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