Lemon Verbena Infusion Consumption Attenuates Oxidative Stress in Dextran Sulfate Sodium-induced Colitis in the Rat

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 2011 Jun 21

PMID 21688009

Citations 17

Authors

Loic Lenoir

Adrien Rossary

Juliette Joubert-Zakeyh

Juliette Vergnaud-Gauduchon

Marie-Chantal Farges

Didier Fraisse

Odile Texier

Jean-Louis Lamaison

Marie-Paule Vasson

Catherine Felgines

Affiliations

Soon will be listed here.

Abstract

Background: Inflammatory bowel diseases (IBD) consist of an uncontrolled intestinal inflammation leading to mucosal disruption. This inflammation is accompanied by an excessive production of reactive oxygen species (ROS). Polyphenols are micronutrients with antioxidative and anti-inflammatory properties, and may play an interesting role in the prevention of intestinal inflammation. Lemon verbena (Aloysia triphylla) infusion is a popular herbal infusion rich in polyphenols (flavones and verbascoside).

Aims: This study evaluated the preventive effects of lemon verbena infusion consumption against mild-to-moderate dextran sulfate sodium (DSS)-induced colitis in rats.

Methods: Wistar rats drank water or lemon verbena infusion for 14 days. On day 15, half of the rats received DSS (4%) in their drink for 7 days. At the end of the experimental period, the colon was taken for histopathological examination and determination of myeloperoxidase (MPO) activity, antioxidant enzyme activities (superoxide dismutase [SOD], glutathione peroxidase [GPx], glutathione reductase [GR], catalase [CAT]), glutathione and lipid peroxidation. Lymphocyte populations were determined in blood, mesenteric nodes and Peyer's patches.

Results: Rats ingested daily 5.6 μmol of polyphenols. DSS reduced food intake and induced colitis, as reflected by histological lesions and increased MPO activity. Although these alterations were not significantly counteracted by lemon verbena consumption, the herbal infusion increased colonic SOD activity and decreased lipid peroxidation (malondialdehyde). Other oxidative stress markers (GPx, GR, CAT, glutathione) were not significantly modified.

Conclusion: Our study shows that the preventive consumption of lemon verbena infusion offered some antioxidative protection during experimental colitis by stimulating SOD activity and decreasing lipid peroxidation.

Citing Articles

Protective effect of aqueous and ethanolic extracts of Kunth on acrylamide-induced neurotoxicity.

Tandisehpanah Z, Foroutanfar A, Aziminia A, Rahbardar M, Razavi B, Hosseinzadeh H Avicenna J Phytomed. 2022; 12(3):281-294.

PMID: 36186928 PMC: 9482716. DOI: 10.22038/AJP.2021.19173.

New App-Based Dietary and Lifestyle Intervention on Weight Loss and Cardiovascular Health.

Martinez-Rodriguez A, Martinez-Olcina M, Mora J, Navarro P, Caturla N, Jones J Sensors (Basel). 2022; 22(3).

PMID: 35161515 PMC: 8840618. DOI: 10.3390/s22030768.

Chemometric Profiling and Bioactivity of Verbena ( ) Methanolic Extract from Four Localities in Tunisia.

Tammar S, Salem N, Wannes W, Limam H, Bourgou S, Fares N Foods. 2021; 10(12).

PMID: 34945462 PMC: 8700817. DOI: 10.3390/foods10122912.

Cytotoxic Effects of Verbascoside on MCF-7 and MDA-MB-231.

Senol H, Tulay P, Ergoren M, Hanoglu A, Calis I, Mocan G Turk J Pharm Sci. 2021; 18(5):637-644.

PMID: 34719192 PMC: 8562110. DOI: 10.4274/tjps.galenos.2021.36599.

Zinc-induced protective effect for testicular ischemia-reperfusion injury by promoting antioxidation via microRNA-101-3p/Nrf2 pathway.

Qin Z, Zhu K, Xue J, Cao P, Xu L, Xu Z Aging (Albany NY). 2019; 11(21):9295-9309.

PMID: 31692452 PMC: 6874427. DOI: 10.18632/aging.102348.

References

Hausmann M, Obermeier F, Paper D, Balan K, Dunger N, Menzel K . In vivo treatment with the herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis. Clin Exp Immunol. 2007; 148(2):373-81. PMC: 1868873. DOI: 10.1111/j.1365-2249.2007.03350.x. View

Oz H, Chen T, McClain C, de Villiers W . Antioxidants as novel therapy in a murine model of colitis. J Nutr Biochem. 2005; 16(5):297-304. DOI: 10.1016/j.jnutbio.2004.09.007. View

Shi X, Winston J, Sarna S . Differential immune and genetic responses in rat models of Crohn's colitis and ulcerative colitis. Am J Physiol Gastrointest Liver Physiol. 2010; 300(1):G41-51. PMC: 3025515. DOI: 10.1152/ajpgi.00358.2010. View

Segui J, Gil F, Gironella M, Alvarez M, Gimeno M, Coronel P . Down-regulation of endothelial adhesion molecules and leukocyte adhesion by treatment with superoxide dismutase is beneficial in chronic immune experimental colitis. Inflamm Bowel Dis. 2005; 11(10):872-82. DOI: 10.1097/01.mib.0000183420.25186.7a. View

Karrasch T, Kim J, Jang B, Jobin C . The flavonoid luteolin worsens chemical-induced colitis in NF-kappaB(EGFP) transgenic mice through blockade of NF-kappaB-dependent protective molecules. PLoS One. 2007; 2(7):e596. PMC: 1895919. DOI: 10.1371/journal.pone.0000596. View

Camuesco D, Galvez J, Nieto A, Comalada M, Rodriguez-Cabezas M, Concha A . Dietary olive oil supplemented with fish oil, rich in EPA and DHA (n-3) polyunsaturated fatty acids, attenuates colonic inflammation in rats with DSS-induced colitis. J Nutr. 2005; 135(4):687-94. DOI: 10.1093/jn/135.4.687. View

Arafa H, Hemeida R, El-Bahrawy A, Hamada F . Prophylactic role of curcumin in dextran sulfate sodium (DSS)-induced ulcerative colitis murine model. Food Chem Toxicol. 2009; 47(6):1311-7. DOI: 10.1016/j.fct.2009.03.003. View

Zamorano-Ponce E, Morales C, Ramos D, Sepulveda C, Cares S, Rivera P . Anti-genotoxic effect of Aloysia triphylla infusion against acrylamide-induced DNA damage as shown by the comet assay technique. Mutat Res. 2006; 603(2):145-50. DOI: 10.1016/j.mrgentox.2005.11.009. View

Vicario M, Amat C, Rivero M, Moreto M, Pelegri C . Dietary glutamine affects mucosal functions in rats with mild DSS-induced colitis. J Nutr. 2007; 137(8):1931-7. DOI: 10.1093/jn/137.8.1931. View

10.

Seidner D, Lashner B, Brzezinski A, Banks P, Goldblum J, Fiocchi C . An oral supplement enriched with fish oil, soluble fiber, and antioxidants for corticosteroid sparing in ulcerative colitis: a randomized, controlled trial. Clin Gastroenterol Hepatol. 2005; 3(4):358-69. DOI: 10.1016/s1542-3565(04)00672-x. View

11.

Cooper H, Murthy S, Shah R, Sedergran D . Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest. 1993; 69(2):238-49. View

12.

Vowinkel T, Kalogeris T, Mori M, Krieglstein C, Granger D . Impact of dextran sulfate sodium load on the severity of inflammation in experimental colitis. Dig Dis Sci. 2004; 49(4):556-64. DOI: 10.1023/b:ddas.0000026298.72088.f7. View

13.

Kruidenier L, Kuiper I, van Duijn W, Mieremet-Ooms M, van Hogezand R, Lamers C . Imbalanced secondary mucosal antioxidant response in inflammatory bowel disease. J Pathol. 2003; 201(1):17-27. DOI: 10.1002/path.1408. View

14.

Koetzner L, Grover G, Boulet J, Jacoby H . Plant-derived polysaccharide supplements inhibit dextran sulfate sodium-induced colitis in the rat. Dig Dis Sci. 2009; 55(5):1278-85. PMC: 2862944. DOI: 10.1007/s10620-009-0848-7. View

15.

Ashokkumar P, Sudhandiran G . Protective role of luteolin on the status of lipid peroxidation and antioxidant defense against azoxymethane-induced experimental colon carcinogenesis. Biomed Pharmacother. 2008; 62(9):590-7. DOI: 10.1016/j.biopha.2008.06.031. View

16.

Mazzon E, Esposito E, Di Paola R, Riccardi L, Caminiti R, Dal Toso R . Effects of verbascoside biotechnologically produced by Syringa vulgaris plant cell cultures in a rodent model of colitis. Naunyn Schmiedebergs Arch Pharmacol. 2009; 380(1):79-94. DOI: 10.1007/s00210-009-0400-5. View

17.

Valentao P, Fernandes E, Carvalho F, Andrade P, Seabra R, de Lourdes Basto M . Studies on the antioxidant activity of Lippia citriodora infusion: scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid. Biol Pharm Bull. 2002; 25(10):1324-7. DOI: 10.1248/bpb.25.1324. View

18.

Gonzalez-Gallego J, Garcia-Mediavilla M, Sanchez-Campos S, Tunon M . Fruit polyphenols, immunity and inflammation. Br J Nutr. 2010; 104 Suppl 3:S15-27. DOI: 10.1017/S0007114510003910. View

19.

Singh K, Jaggi A, Singh N . Exploring the ameliorative potential of Punica granatum in dextran sulfate sodium induced ulcerative colitis in mice. Phytother Res. 2009; 23(11):1565-74. DOI: 10.1002/ptr.2822. View

20.

Koutroubakis I, Malliaraki N, Dimoulios P, Karmiris K, Castanas E, Kouroumalis E . Decreased total and corrected antioxidant capacity in patients with inflammatory bowel disease. Dig Dis Sci. 2004; 49(9):1433-7. DOI: 10.1023/b:ddas.0000042242.22898.d9. View