Zinc-induced Protective Effect for Testicular Ischemia-reperfusion Injury by Promoting Antioxidation Via MicroRNA-101-3p/Nrf2 Pathway

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2019 Nov 7

PMID 31692452

Citations 7

Authors

Zhiqiang Qin

Kai Zhu

Jianxin Xue

Pu Cao

Luwei Xu

Zheng Xu

Kai Liang

Jiageng Zhu

Ruipeng Jia

Affiliations

Soon will be listed here.

Abstract

The present study was performed to determine the protective effect of Zinc on the rat testicular ischemia-reperfusion (I/R) injury and its mechanism. , the pathological changes and the apoptosis index were significantly relieved in the rats with Low-dose Zinc pretreatment, compared to the I/R group. After Low-dose Zinc treatment, the levels of tissue Malondialdehyde (MDA) were significantly decreased, while tissue antioxidant indices were significantly increased. Meanwhile, the level of NF-κB was significantly lower compared to I/R group, while the levels of Nrf2-dependent antioxidant enzymes were significantly higher in Low-dose Zinc+I/R group. , Low-dose Zinc markedly increased Leydig cell (TM3) cell viability, and relieved testicular oxidative damage via down-regulating ROS. A total of 22 differently expressed microRNAs were screened out using microRNA microarray in rat testicular tissue caused by I/R injury, especially showing that miR-101-3p was selected as the target miRNA. Furthermore, the levels of Nrf2 and NF-κB were apparently increased/decreased in TM3 cells treated with Hypoxic/Reoxygenation (H/R) after miR-101-3p mimics/inhibitor. In addition, H/R-induced testicular oxidative damage was recovered in TM3 administrated with miR-101-3p inhibitor and si-Nrf2. Therefore, this study provided a novel insight for investigating protective effect of Zinc on testicular I/R injury by promoting antioxidation via miR-101-3p/Nrf2.

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References

Bei Y, Pan L, Zhou Q, Zhao C, Xie Y, Wu C . Cathelicidin-related antimicrobial peptide protects against myocardial ischemia/reperfusion injury. BMC Med. 2019; 17(1):42. PMC: 6381635. DOI: 10.1186/s12916-019-1268-y. View

Zhai X, Chen X, Shi J, Shi D, Ye Z, Liu W . Lactulose ameliorates cerebral ischemia-reperfusion injury in rats by inducing hydrogen by activating Nrf2 expression. Free Radic Biol Med. 2013; 65:731-741. DOI: 10.1016/j.freeradbiomed.2013.08.004. View

Miao X, Bai Y, Sun W, Cui W, Xin Y, Wang Y . Sulforaphane prevention of diabetes-induced aortic damage was associated with the up-regulation of Nrf2 and its down-stream antioxidants. Nutr Metab (Lond). 2012; 9(1):84. PMC: 3495894. DOI: 10.1186/1743-7075-9-84. View

Liu H, Wang L, Weng X, Chen H, Du Y, Diao C . Inhibition of Brd4 alleviates renal ischemia/reperfusion injury-induced apoptosis and endoplasmic reticulum stress by blocking FoxO4-mediated oxidative stress. Redox Biol. 2019; 24:101195. PMC: 6475721. DOI: 10.1016/j.redox.2019.101195. View

Zhang Q, Sun H, Zheng R, Li Y, Zhang Q, Cheng P . Meta-analysis of microRNA-183 family expression in human cancer studies comparing cancer tissues with noncancerous tissues. Gene. 2013; 527(1):26-32. DOI: 10.1016/j.gene.2013.06.006. View

Ozyildirim S, Baltaci A, Sahna E, Mogulkoc R . Effects of Chronic and Acute Zinc Supplementation on Myocardial Ischemia-Reperfusion Injury in Rats. Biol Trace Elem Res. 2016; 178(1):64-70. DOI: 10.1007/s12011-016-0903-0. View

Jurowski K, Szewczyk B, Nowak G, Piekoszewski W . Biological consequences of zinc deficiency in the pathomechanisms of selected diseases. J Biol Inorg Chem. 2014; 19(7):1069-79. PMC: 4175048. DOI: 10.1007/s00775-014-1139-0. View

Wen D, Zou Y, Gao Y, Zhao Q, Xie Y, Shen P . Inhibitor of DNA Binding 1 Is Induced during Kidney Ischemia-Reperfusion and Is Critical for the Induction of Hypoxia-Inducible Factor-1α. Biomed Res Int. 2016; 2016:4634386. PMC: 4835634. DOI: 10.1155/2016/4634386. View

Jaiswal A . Nrf2 signaling in coordinated activation of antioxidant gene expression. Free Radic Biol Med. 2004; 36(10):1199-207. DOI: 10.1016/j.freeradbiomed.2004.02.074. View

10.

Wu D, Chen X, Ding R, Qiao X, Shi S, Xie Y . Ischemia/reperfusion induce renal tubule apoptosis by inositol 1,4,5-trisphosphate receptor and L-type Ca2+ channel opening. Am J Nephrol. 2008; 28(3):487-99. DOI: 10.1159/000113107. View

11.

Nowak G, Siwek M, Dudek D, Zieba A, Pilc A . Effect of zinc supplementation on antidepressant therapy in unipolar depression: a preliminary placebo-controlled study. Pol J Pharmacol. 2004; 55(6):1143-7. View

12.

Hoesel B, Schmid J . The complexity of NF-κB signaling in inflammation and cancer. Mol Cancer. 2013; 12:86. PMC: 3750319. DOI: 10.1186/1476-4598-12-86. View

13.

Bi J, Zhang J, Ren Y, Du Z, Li Q, Wang Y . Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative stress. Redox Biol. 2018; 20:296-306. PMC: 6216086. DOI: 10.1016/j.redox.2018.10.019. View

14.

Ho E . Zinc deficiency, DNA damage and cancer risk. J Nutr Biochem. 2004; 15(10):572-8. DOI: 10.1016/j.jnutbio.2004.07.005. View

15.

Hur G, Ryu Y, Yun H, Jeon B, Kim Y, Seok J . Hepatic ischemia/reperfusion in rats induces iNOS gene transcription by activation of NF-kappaB. Biochem Biophys Res Commun. 1999; 261(3):917-22. DOI: 10.1006/bbrc.1999.1143. View

16.

Lenoir L, Rossary A, Joubert-Zakeyh J, Vergnaud-Gauduchon J, Farges M, Fraisse D . Lemon verbena infusion consumption attenuates oxidative stress in dextran sulfate sodium-induced colitis in the rat. Dig Dis Sci. 2011; 56(12):3534-45. DOI: 10.1007/s10620-011-1784-x. View

17.

Reilly P, Schiller H, Bulkley G . Pharmacologic approach to tissue injury mediated by free radicals and other reactive oxygen metabolites. Am J Surg. 1991; 161(4):488-503. DOI: 10.1016/0002-9610(91)91120-8. View

18.

Granger D, Korthuis R . Physiologic mechanisms of postischemic tissue injury. Annu Rev Physiol. 1995; 57:311-32. DOI: 10.1146/annurev.ph.57.030195.001523. View

19.

Gupta R, Patel A, Shah N, Chaudhary A, Jha U, Yadav U . Oxidative stress and antioxidants in disease and cancer: a review. Asian Pac J Cancer Prev. 2014; 15(11):4405-9. DOI: 10.7314/apjcp.2014.15.11.4405. View

20.

Ozgur B, Telli O, Yuceturk C, Sarici H, Ozer E, Surer H . The effect of sildenafil and udenafil on testicular damage following ischemia-reperfusion injury in rats. J Urol. 2014; 192(4):1272-7. DOI: 10.1016/j.juro.2014.04.011. View