Metabolaid Combination of Lemon Verbena and Hibiscus Flower Extract Prevents High-Fat Diet-Induced Obesity Through AMP-Activated Protein Kinase Activation

Overview

Journal Nutrients

Date 2018 Sep 12

PMID 30200432

Citations 18

Authors

Young-Sil Lee

Won-Kyung Yang

Hwa Yeon Kim

Bokkee Min

Nuria Caturla

Jonathan Jones

Yang-Chun Park

Young-Cheol Lee

Seung-Hyung Kim

Affiliations

Soon will be listed here.

Abstract

Lemon verbena () has been used as a food spice, cosmetic, and in traditional medicine formulations to treat asthma and diabetes in South America and Southern Europe. Hibiscus flower ( L.) is used in traditional Chinese medicine in the form of a tea to treat hypertension and inflammation. In the present study, we examined the synergistic effects of a formula of Metabolaid (MetA), a combination of lemon verbena and hibiscus-flower extracts, on obesity and its complications in high-fat-diet (HFD)-induced obese mice. The results showed that MetA decreased body weight, white adipose tissue (WAT), and liver weight. Additionally, serum and hepatic lipid profiles, glucose levels, glucose tolerance, and cold-induced thermogenesis were significantly improved. Appetite-regulating hormones adiponectin and leptin were significantly increased and decreased, respectively, while the inflammatory-related factors tumor necrosis factor (TNF)-α and interleukin (IL)-6 were downregulated by MetA. Adipogenesis-activating gene expression was decreased, while increased thermogenesis-inducing genes were upregulated in the WAT, correlating with increased phosphorylation of AMPK and fatty-acid oxidation in the liver. Taken together, these results suggest that MetA decreased obesity and its complications in HFD mice. Therefore, this formula may be a candidate for the prevention and treatment of obesity and its complications.

Citing Articles

Research advances in the therapy of metabolic syndrome.

Lin Z, Sun L Front Pharmacol. 2024; 15:1364881.

PMID: 39139641 PMC: 11319131. DOI: 10.3389/fphar.2024.1364881.

Comparison of chitosan nanoparticles containing Lippia citriodora essential oil and citral on the induction of apoptosis in A375 melanoma cells.

Karimivaselabadi A, Osanloo M, Ghanbariasad A, Zarenezhad E, Hosseini H BMC Complement Med Ther. 2023; 23(1):435.

PMID: 38041055 PMC: 10691079. DOI: 10.1186/s12906-023-04268-2.

Efficacy of Carotenoid-Loaded Gelatin Nanoparticles in Reducing Plasma Cytokines and Adipocyte Hypertrophy in Wistar Rats.

de Queiroz J, Medeiros I, Lima M, Carvalho F, Camillo C, Santos P Int J Mol Sci. 2023; 24(13).

PMID: 37445834 PMC: 10341683. DOI: 10.3390/ijms241310657.

: An Unconventional Alternative Edible Flower Rich in Bioactive Compounds.

Dos Santos Silva L, Ramos A, Cavalcante D, Kinupp V, Rodrigues J, Ventura B Molecules. 2023; 28(12).

PMID: 37375373 PMC: 10305401. DOI: 10.3390/molecules28124819.

Anti-inflammatory agents as modulators of the inflammation in adipose tissue: A systematic review.

Nascimento S, Carvalho de Queiroz J, de Medeiros A, de Franca Nunes A, Piuvezam G, Maciel B PLoS One. 2022; 17(9):e0273942.

PMID: 36048868 PMC: 9436134. DOI: 10.1371/journal.pone.0273942.

References

Bracale R, Petroni M, Davinelli S, Bracale U, Scapagnini G, Carruba M . Muscle uncoupling protein 3 expression is unchanged by chronic ephedrine/caffeine treatment: results of a double blind, randomised clinical trial in morbidly obese females. PLoS One. 2014; 9(6):e98244. PMC: 4048162. DOI: 10.1371/journal.pone.0098244. View

Hotamisligil G, Shargill N, Spiegelman B . Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science. 1993; 259(5091):87-91. DOI: 10.1126/science.7678183. View

Rios-Hoyo A, Gutierrez-Salmean G . New Dietary Supplements for Obesity: What We Currently Know. Curr Obes Rep. 2016; 5(2):262-70. DOI: 10.1007/s13679-016-0214-y. View

Rashid S, Watanabe T, Sakaue T, Lewis G . Mechanisms of HDL lowering in insulin resistant, hypertriglyceridemic states: the combined effect of HDL triglyceride enrichment and elevated hepatic lipase activity. Clin Biochem. 2003; 36(6):421-9. DOI: 10.1016/s0009-9120(03)00078-x. View

Fried S, Ricci M, Russell C, Laferrere B . Regulation of leptin production in humans. J Nutr. 2001; 130(12):3127S-3131S. DOI: 10.1093/jn/130.12.3127S. View

Spiegelman B, Flier J . Obesity and the regulation of energy balance. Cell. 2001; 104(4):531-43. DOI: 10.1016/s0092-8674(01)00240-9. View

Hansawasdi C, Kawabata J, Kasai T . Hibiscus acid as an inhibitor of starch digestion in the Caco-2 cell model system. Biosci Biotechnol Biochem. 2001; 65(9):2087-9. DOI: 10.1271/bbb.65.2087. View

Lenoir L, Rossary A, Joubert-Zakeyh J, Vergnaud-Gauduchon J, Farges M, Fraisse D . Lemon verbena infusion consumption attenuates oxidative stress in dextran sulfate sodium-induced colitis in the rat. Dig Dis Sci. 2011; 56(12):3534-45. DOI: 10.1007/s10620-011-1784-x. View

Zhang B, Zhou G, Li C . AMPK: an emerging drug target for diabetes and the metabolic syndrome. Cell Metab. 2009; 9(5):407-16. DOI: 10.1016/j.cmet.2009.03.012. View

10.

Bahramsoltani R, Rostamiasrabadi P, Shahpiri Z, Marques A, Rahimi R, Farzaei M . Aloysia citrodora Paláu (Lemon verbena): A review of phytochemistry and pharmacology. J Ethnopharmacol. 2018; 222:34-51. DOI: 10.1016/j.jep.2018.04.021. View

11.

Boix-Castejon M, Herranz-Lopez M, Gago A, Olivares-Vicente M, Caturla N, Roche E . Hibiscus and lemon verbena polyphenols modulate appetite-related biomarkers in overweight subjects: a randomized controlled trial. Food Funct. 2018; 9(6):3173-3184. DOI: 10.1039/c8fo00367j. View

12.

Ahima R . Adipose tissue as an endocrine organ. Obesity (Silver Spring). 2006; 14 Suppl 5:242S-249S. DOI: 10.1038/oby.2006.317. View

13.

Cristancho A, Lazar M . Forming functional fat: a growing understanding of adipocyte differentiation. Nat Rev Mol Cell Biol. 2011; 12(11):722-34. PMC: 7171550. DOI: 10.1038/nrm3198. View

14.

Wu J, Bostrom P, Sparks L, Ye L, Choi J, Giang A . Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell. 2012; 150(2):366-76. PMC: 3402601. DOI: 10.1016/j.cell.2012.05.016. View

15.

Di Giancamillo A, Rossi R, Pastorelli G, Deponti D, Carollo V, Casamassima D . The effects of dietary verbascoside on blood and liver oxidative stress status induced by a high n-6 polyunsaturated fatty acids diet in piglets. J Anim Sci. 2015; 93(6):2849-59. DOI: 10.2527/jas.2014-8607. View

16.

Haji Faraji M, Haji Tarkhani A . The effect of sour tea (Hibiscus sabdariffa) on essential hypertension. J Ethnopharmacol. 1999; 65(3):231-6. DOI: 10.1016/s0378-8741(98)00157-3. View

17.

Lagathu C, Bastard J, Auclair M, Maachi M, Capeau J, Caron M . Chronic interleukin-6 (IL-6) treatment increased IL-6 secretion and induced insulin resistance in adipocyte: prevention by rosiglitazone. Biochem Biophys Res Commun. 2003; 311(2):372-9. DOI: 10.1016/j.bbrc.2003.10.013. View

18.

Kim M, Kim J, Kim H, Moon S, Shin B, Park K . Hibiscus extract inhibits the lipid droplet accumulation and adipogenic transcription factors expression of 3T3-L1 preadipocytes. J Altern Complement Med. 2003; 9(4):499-504. DOI: 10.1089/107555303322284785. View

19.

Corbi G, Conti V, Komici K, Manzo V, Filippelli A, Palazzo M . Phenolic Plant Extracts Induce Sirt1 Activity and Increase Antioxidant Levels in the Rabbit's Heart and Liver. Oxid Med Cell Longev. 2018; 2018:2731289. PMC: 6079382. DOI: 10.1155/2018/2731289. View

20.

Jamila F, Mostafa E . Ethnobotanical survey of medicinal plants used by people in Oriental Morocco to manage various ailments. J Ethnopharmacol. 2014; 154(1):76-87. DOI: 10.1016/j.jep.2014.03.016. View