No Effect of Pertussis Toxin on Peripheral Prejunctional Alpha 2-adrenoceptor-mediated Responses and on Endothelium-dependent Relaxations in the Rat

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1990 Jun 1

PMID 2165838

Citations 4

Authors

J R Docherty

Affiliations

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Abstract

1. We have investigated the effects of pertussis toxin treatment on a variety of peripheral tissues in the rat. 2. Incubation with pertussis toxin (1 microgram ml-1) in vitro failed to alter the negative inotropic actions of acetylcholine in rat left atria. 3. Pretreatment with pertussis toxin (6 micrograms kg-1, i.v., 3-4 days) abolished the negative inotropic actions of acetylcholine in rat left atria. 4. Pretreatment with pertussis toxin (40 micrograms kg-1, i.v., 3-4 days) failed to alter the prejunctional inhibitory actions of the alpha 2-adrenoceptor agonist xylazine, either in terms of the isometric contraction to a single stimulus in rat vas deferens or in terms of stimulation-evoked overflow of tritium in atria pre-incubated with [3H]-noradrenaline. 5. Pretreatment with pertussis toxin (6 micrograms kg-1, i.v., 3-4 days) failed to affect, and pertussis toxin (40 micrograms kg-1, i.v., 3-4 days) potentiated endothelium-dependent relaxations of rat aorta to histamine and acetylcholine. 6. It seems unlikely that peripheral prejunctional actions of alpha 2-adrenoceptor agonists or endothelium-dependent relaxations of rat aorta involve pertussis toxin-sensitive G proteins.

Citing Articles

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Involvement of pertussis toxin-sensitive and -insensitive mechanisms in alpha-adrenoceptor modulation of noradrenaline release from rat sympathetic neurones in tissue culture.

Hill C, Powis D, Hendry I Br J Pharmacol. 1993; 110(1):281-8.

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Prejunctional alpha 2-adrenoceptors in mouse atria function through G-proteins which are sensitive to N-ethylmaleimide, but not pertussis toxin.

Murphy T, Foucart S, Majewski H Br J Pharmacol. 1992; 106(4):871-6.

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Pertussis toxin abolishes the inhibition of Ca2+ currents and of noradrenaline release via alpha 2-adrenoceptors in chick sympathetic neurons.

Boehm S, Huck S, Drobny H, Singer E Naunyn Schmiedebergs Arch Pharmacol. 1992; 345(5):606-9.

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