SLAIN2 Links Microtubule Plus End-tracking Proteins and Controls Microtubule Growth in Interphase

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2011 Jun 8

PMID 21646404

Citations 76

Authors

Babet van der Vaart

Cristina Manatschal

Ilya Grigoriev

Vincent Olieric

Susana Montenegro Gouveia

Sasa Bjelic

Jeroen Demmers

Ivan Vorobjev

Casper C Hoogenraad

Michel O Steinmetz

Anna Akhmanova

Affiliations

Soon will be listed here.

Abstract

The ends of growing microtubules (MTs) accumulate a set of diverse factors known as MT plus end-tracking proteins (+TIPs), which control microtubule dynamics and organization. In this paper, we identify SLAIN2 as a key component of +TIP interaction networks. We showed that the C-terminal part of SLAIN2 bound to end-binding proteins (EBs), cytoplasmic linker proteins (CLIPs), and CLIP-associated proteins and characterized in detail the interaction of SLAIN2 with EB1 and CLIP-170. Furthermore, we found that the N-terminal part of SLAIN2 interacted with ch-TOG, the mammalian homologue of the MT polymerase XMAP215. Through its multiple interactions, SLAIN2 enhanced ch-TOG accumulation at MT plus ends and, as a consequence, strongly stimulated processive MT polymerization in interphase cells. Depletion or disruption of the SLAIN2-ch-TOG complex led to disorganization of the radial MT array. During mitosis, SLAIN2 became highly phosphorylated, and its interaction with EBs and ch-TOG was inhibited. Our study provides new insights into the molecular mechanisms underlying cell cycle-specific regulation of MT polymerization and the organization of the MT network.

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