Transient Dominant Selection of Recombinant Vaccinia Viruses

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1990 Jun 1

PMID 2159565

Citations 151

Authors

F G Falkner

B Moss

Affiliations

Soon will be listed here.

Abstract

A general method for constructing and selecting recombinant vaccinia viruses with insertions, deletions, or mutations in any gene that is similar in principle to one originally devised for Saccharomyces cerevisiae (S. Scherer and R. W. Davis, Proc. Natl. Acad. Sci. USA 76:4951-4955, 1979) is described. The selectable marker used, Escherichia coli guanine phosphoribosyltransferase, is not retained within the final recombinant virus, and hence, this procedure may be used serially to introduce several foreign genes or to make multiple site-directed mutations.

Citing Articles

Experimental Evolution of Poxviruses.

Banerjee S, Jewell K, Brennan G Methods Mol Biol. 2024; 2860:241-256.

PMID: 39621272 DOI: 10.1007/978-1-0716-4160-6_16.

Filamin B restricts vaccinia virus spread and is targeted by vaccinia virus protein C4.

Georgana I, Scutts S, Gao C, Lu Y, Torres A, Ren H J Virol. 2024; 98(3):e0148523.

PMID: 38412044 PMC: 10949515. DOI: 10.1128/jvi.01485-23.

Rendezvous with Vaccinia Virus in the Post-smallpox Era: R&D Advances.

Wang Y Viruses. 2023; 15(8).

PMID: 37632084 PMC: 10457812. DOI: 10.3390/v15081742.

CRISPR-mediated rapid arming of poxvirus vectors enables facile generation of the novel immunotherapeutic STINGPOX.

Whelan J, Singaravelu R, Wang F, Pelin A, Tamming L, Pugliese G Front Immunol. 2023; 13:1050250.

PMID: 36713447 PMC: 9880309. DOI: 10.3389/fimmu.2022.1050250.

Lumpy Skin Disease Virus with Four Knocked Out Genes Was Attenuated In Vivo and Protects Cattle from Infection.

Chervyakova O, Issabek A, Sultankulova K, Bopi A, Kozhabergenov N, Omarova Z Vaccines (Basel). 2022; 10(10).

PMID: 36298570 PMC: 9610274. DOI: 10.3390/vaccines10101705.

References

Mulligan R, Berg P . Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc Natl Acad Sci U S A. 1981; 78(4):2072-6. PMC: 319285. DOI: 10.1073/pnas.78.4.2072. View

Spehner D, Drillien R, Lecocq J . Construction of fowlpox virus vectors with intergenic insertions: expression of the beta-galactosidase gene and the measles virus fusion gene. J Virol. 1990; 64(2):527-33. PMC: 249140. DOI: 10.1128/JVI.64.2.527-533.1990. View

St John T, Scherer S, McDonell M, Davis R . Deletion analysis of the Saccharomyces GAL gene cluster. Transcription from three promoters. J Mol Biol. 1981; 152(2):317-34. DOI: 10.1016/0022-2836(81)90245-x. View

Weir J, Bajszar G, Moss B . Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell-free translation of selected mRNA. Proc Natl Acad Sci U S A. 1982; 79(4):1210-4. PMC: 345931. DOI: 10.1073/pnas.79.4.1210. View

Scherer S, Davis R . Replacement of chromosome segments with altered DNA sequences constructed in vitro. Proc Natl Acad Sci U S A. 1979; 76(10):4951-5. PMC: 413056. DOI: 10.1073/pnas.76.10.4951. View

Panicali D, Paoletti E . Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Proc Natl Acad Sci U S A. 1982; 79(16):4927-31. PMC: 346798. DOI: 10.1073/pnas.79.16.4927. View

Mackett M, Smith G, Moss B . Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proc Natl Acad Sci U S A. 1982; 79(23):7415-9. PMC: 347350. DOI: 10.1073/pnas.79.23.7415. View

Mackett M, Smith G, Moss B . General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes. J Virol. 1984; 49(3):857-64. PMC: 255547. DOI: 10.1128/JVI.49.3.857-864.1984. View

Cochran M, Puckett C, Moss B . In vitro mutagenesis of the promoter region for a vaccinia virus gene: evidence for tandem early and late regulatory signals. J Virol. 1985; 54(1):30-7. PMC: 254756. DOI: 10.1128/JVI.54.1.30-37.1985. View

10.

Bertholet C, Drillien R, Wittek R . One hundred base pairs of 5' flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription. Proc Natl Acad Sci U S A. 1985; 82(7):2096-100. PMC: 397499. DOI: 10.1073/pnas.82.7.2096. View

11.

Shida H . Nucleotide sequence of the vaccinia virus hemagglutinin gene. Virology. 1986; 150(2):451-62. DOI: 10.1016/0042-6822(86)90309-0. View

12.

Chakrabarti S, Brechling K, Moss B . Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques. Mol Cell Biol. 1985; 5(12):3403-9. PMC: 369169. DOI: 10.1128/mcb.5.12.3403-3409.1985. View

13.

Franke C, Rice C, Strauss J, Hruby D . Neomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants. Mol Cell Biol. 1985; 5(8):1918-24. PMC: 366908. DOI: 10.1128/mcb.5.8.1918-1924.1985. View

14.

Panicali D, Grzelecki A, Huang C . Vaccinia virus vectors utilizing the beta-galactosidase assay for rapid selection of recombinant viruses and measurement of gene expression. Gene. 1986; 47(2-3):193-9. DOI: 10.1016/0378-1119(86)90063-6. View

15.

Ball L . High-frequency homologous recombination in vaccinia virus DNA. J Virol. 1987; 61(6):1788-95. PMC: 254181. DOI: 10.1128/JVI.61.6.1788-1795.1987. View

16.

Evans D, Stuart D, McFadden G . High levels of genetic recombination among cotransfected plasmid DNAs in poxvirus-infected mammalian cells. J Virol. 1988; 62(2):367-75. PMC: 250545. DOI: 10.1128/JVI.62.2.367-375.1988. View

17.

Spyropoulos D, Roberts B, Panicali D, Cohen L . Delineation of the viral products of recombination in vaccinia virus-infected cells. J Virol. 1988; 62(3):1046-54. PMC: 253665. DOI: 10.1128/JVI.62.3.1046-1054.1988. View

18.

Falkner F, Moss B . Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors. J Virol. 1988; 62(6):1849-54. PMC: 253265. DOI: 10.1128/JVI.62.6.1849-1854.1988. View

19.

Boyle D, Coupar B . A dominant selectable marker for the construction of recombinant poxviruses. Gene. 1988; 65(1):123-8. DOI: 10.1016/0378-1119(88)90424-6. View

20.

Rodriguez J, Esteban M . Plaque size phenotype as a selectable marker to generate vaccinia virus recombinants. J Virol. 1989; 63(2):997-1001. PMC: 247783. DOI: 10.1128/JVI.63.2.997-1001.1989. View