Filamin B Restricts Vaccinia Virus Spread and is Targeted by Vaccinia Virus Protein C4

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2024 Feb 27

PMID 38412044

Authors

Iliana Georgana

Simon R Scutts

Chen Gao

Yongxu Lu

Alice A Torres

Hongwei Ren

Edward Emmott

Jinghao Men

Keefe Oei

Geoffrey L Smith

Affiliations

Soon will be listed here.

Abstract

Importance: Vaccinia virus (VACV), the vaccine against smallpox and monkeypox, encodes many proteins to counteract the host immune response. Investigating these proteins provides insights into viral immune evasion mechanisms and thereby indicates how to engineer safer and more immunogenic VACV-based vaccines. Here, we report that the N-terminal domain of VACV protein C4 interacts directly with the cytoskeletal protein filamin B (FLNB), and this domain of C4 contributes to virus virulence. Furthermore, VACV replicates and spreads better in cells lacking FLNB, thus demonstrating that FLNB has antiviral activity. VACV utilizes the cytoskeleton for movement within and between cells; however, previous studies show no involvement of C4 in VACV replication or spread. Thus, C4 associates with FLNB for a different reason, suggesting that the cytoskeleton has further uncharacterized roles during virus infection.

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