Genetic Analysis of Basophil Function in Vivo

Overview

Journal Nat Immunol

Date 2011 May 10

PMID 21552267

Citations 143

Authors

Brandon M Sullivan

Hong-Erh Liang

Jennifer K Bando

Davina Wu

Laurence E Cheng

James K McKerrow

Christopher D C Allen

Richard M Locksley

Affiliations

Soon will be listed here.

Abstract

Contributions by basophils to allergic and helminth immunity remain incompletely defined. Using sensitive interleukin 4 (Il4) reporter alleles, we demonstrate here that basophil IL-4 production occurs by a CD4(+) T cell-dependent process restricted to the peripheral tissues affected. We genetically marked and achieved specific deletion of basophils and found that basophils did not mediate T helper type 2 (T(H)2) priming in vivo. Two-photon imaging confirmed that basophils did not interact with antigen-specific T cells in lymph nodes but engaged in prolonged serial interactions with T cells in lung tissues. Although targeted deletion of IL-4 and IL-13 in either CD4(+) T cells or basophils had a minimal effect on worm clearance, deletion from both lineages demonstrated a nonredundant role for basophil cytokines in primary helminth immunity.

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