Ultra-performance Ion-pairing Liquid Chromatography with On-line Electrospray Ion Trap Mass Spectrometry for Heparin Disaccharide Analysis

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 2011 May 3

PMID 21530482

Citations 34

Authors

Bo Yang

Amanda Weyers

Jong Youn Baik

Eric Sterner

Susan Sharfstein

Shaker A Mousa

Fuming Zhang

Jonathan S Dordick

Robert J Linhardt

Affiliations

Soon will be listed here.

Abstract

A high-resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultra-performance liquid chromatography in a reverse-phase ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues, and biological fluids, because it provides high sensitivity without being subject to interference from proteins, peptides, and other sample impurities.

Citing Articles

[Advances in heparin structural analysis by chromatography technologies].

Ouyang Y, Yi L, Qiu L, Zhang Z Se Pu. 2023; 41(2):107-121.

PMID: 36725707 PMC: 9892979. DOI: 10.3724/SP.J.1123.2022.07020.

Recent advances in biotechnology for heparin and heparan sulfate analysis.

Qiao M, Lin L, Xia K, Li J, Zhang X, Linhardt R Talanta. 2020; 219:121270.

PMID: 32887160 PMC: 7474733. DOI: 10.1016/j.talanta.2020.121270.

Online Capillary Zone Electrophoresis Negative Electron Transfer Dissociation Tandem Mass Spectrometry of Glycosaminoglycan Mixtures.

Stickney M, Sanderson P, Leach 3rd F, Zhang F, Linhardt R, Jonathan Amster I Int J Mass Spectrom. 2020; 445.

PMID: 32641905 PMC: 7343235. DOI: 10.1016/j.ijms.2019.116209.

Analysis of the Glycosaminoglycan Chains of Proteoglycans.

Song Y, Zhang F, Linhardt R J Histochem Cytochem. 2020; 69(2):121-135.

PMID: 32623943 PMC: 7841699. DOI: 10.1369/0022155420937154.

Enzyme immobilization offers a robust tool to scale up the production of longer, diverse, natural glycosaminoglycan oligosaccharides.

Alabbas A, Desai U Glycobiology. 2020; 30(10):768-773.

PMID: 32193533 PMC: 7526740. DOI: 10.1093/glycob/cwaa027.

References

Muszbek L, Bereczky Z, Kovacs B, Komaromi I . Antithrombin deficiency and its laboratory diagnosis. Clin Chem Lab Med. 2010; 48 Suppl 1:S67-78. DOI: 10.1515/CCLM.2010.368. View

Yamada S, Morimoto H, Fujisawa T, Sugahara K . Glycosaminoglycans in Hydra magnipapillata (Hydrozoa, Cnidaria): demonstration of chondroitin in the developing nematocyst, the sting organelle, and structural characterization of glycosaminoglycans. Glycobiology. 2007; 17(8):886-94. DOI: 10.1093/glycob/cwm051. View

Volpi N, Linhardt R . High-performance liquid chromatography-mass spectrometry for mapping and sequencing glycosaminoglycan-derived oligosaccharides. Nat Protoc. 2010; 5(6):993-1004. PMC: 3359136. DOI: 10.1038/nprot.2010.48. View

Li B, Ryan P, Ray B, Leister K, Sirimuthu N, Ryder A . Rapid characterization and quality control of complex cell culture media solutions using raman spectroscopy and chemometrics. Biotechnol Bioeng. 2010; 107(2):290-301. DOI: 10.1002/bit.22813. View

Zhang Z, Li B, Suwan J, Zhang F, Wang Z, Liu H . Analysis of pharmaceutical heparins and potential contaminants using (1)H-NMR and PAGE. J Pharm Sci. 2009; 98(11):4017-26. DOI: 10.1002/jps.21729. View

Linhardt R . 2003 Claude S. Hudson Award address in carbohydrate chemistry. Heparin: structure and activity. J Med Chem. 2003; 46(13):2551-64. DOI: 10.1021/jm030176m. View

Solakyildirim K, Zhang Z, Linhardt R . Ultraperformance liquid chromatography with electrospray ionization ion trap mass spectrometry for chondroitin disaccharide analysis. Anal Biochem. 2009; 397(1):24-8. PMC: 2919804. DOI: 10.1016/j.ab.2009.09.031. View

Cecchi T, Pucciarelli F, Passamonti P . Extended thermodynamic approach to ion interaction chromatography. Anal Chem. 2001; 73(11):2632-9. DOI: 10.1021/ac001341y. View

Lawrence R, Olson S, Steele R, Wang L, Warrior R, Cummings R . Evolutionary differences in glycosaminoglycan fine structure detected by quantitative glycan reductive isotope labeling. J Biol Chem. 2008; 283(48):33674-84. PMC: 2586254. DOI: 10.1074/jbc.M804288200. View

10.

Zhang Z, Xie J, Liu H, Liu J, Linhardt R . Quantification of heparan sulfate disaccharides using ion-pairing reversed-phase microflow high-performance liquid chromatography with electrospray ionization trap mass spectrometry. Anal Chem. 2009; 81(11):4349-55. PMC: 6516462. DOI: 10.1021/ac9001707. View

11.

Chuang W, McAllister H, Rabenstein L . Chromatographic methods for product-profile analysis and isolation of oligosaccharides produced by heparinase-catalyzed depolymerization of heparin. J Chromatogr A. 2001; 932(1-2):65-74. DOI: 10.1016/s0021-9673(01)01241-9. View

12.

Guerrini M, Beccati D, Shriver Z, Naggi A, Viswanathan K, Bisio A . Oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events. Nat Biotechnol. 2008; 26(6):669-75. PMC: 3491566. DOI: 10.1038/nbt1407. View

13.

Cecchi T, Passamonti P . Retention mechanism for ion-pair chromatography with chaotropic reagents. J Chromatogr A. 2008; 1216(10):1789-97. DOI: 10.1016/j.chroma.2008.10.031. View

14.

Brustkern A, Buhse L, Nasr M, Al-Hakim A, Keire D . Characterization of currently marketed heparin products: reversed-phase ion-pairing liquid chromatography mass spectrometry of heparin digests. Anal Chem. 2010; 82(23):9865-70. DOI: 10.1021/ac102301j. View

15.

Jones C, Membreno N, Larive C . Insights into the mechanism of separation of heparin and heparan sulfate disaccharides by reverse-phase ion-pair chromatography. J Chromatogr A. 2009; 1217(4):479-88. DOI: 10.1016/j.chroma.2009.11.064. View

16.

Jandik K, Kruep D, Cartier M, Linhardt R . Accelerated stability studies of heparin. J Pharm Sci. 1996; 85(1):45-51. DOI: 10.1021/js9502736. View

17.

Chai W, Hounsell E, Bauer C, Lawson A . Characterisation by LSI-MS and 1H NMR spectroscopy of tetra-, hexa-, and octa-saccharides of porcine intestinal heparin. Carbohydr Res. 1995; 269(1):139-56. DOI: 10.1016/0008-6215(94)00349-k. View

18.

Shaya D, Tocilj A, Li Y, Myette J, Venkataraman G, Sasisekharan R . Crystal structure of heparinase II from Pedobacter heparinus and its complex with a disaccharide product. J Biol Chem. 2006; 281(22):15525-35. DOI: 10.1074/jbc.M512055200. View

19.

Tumova S, Woods A, Couchman J . Heparan sulfate proteoglycans on the cell surface: versatile coordinators of cellular functions. Int J Biochem Cell Biol. 2000; 32(3):269-88. DOI: 10.1016/s1357-2725(99)00116-8. View

20.

Wang L, Fuster M, Sriramarao P, Esko J . Endothelial heparan sulfate deficiency impairs L-selectin- and chemokine-mediated neutrophil trafficking during inflammatory responses. Nat Immunol. 2005; 6(9):902-10. DOI: 10.1038/ni1233. View