Distinct Properties of the XY Pseudoautosomal Region Crucial for Male Meiosis

Overview

Journal Science

Specialty Science

Date 2011 Feb 19

PMID 21330546

Citations 149

Authors

Liisa Kauppi

Marco Barchi

Frederic Baudat

Peter J Romanienko

Scott Keeney

Maria Jasin

Affiliations

Soon will be listed here.

Abstract

Meiosis requires that each chromosome find its homologous partner and undergo at least one crossover. X-Y chromosome segregation hinges on efficient crossing-over in a very small region of homology, the pseudoautosomal region (PAR). We find that mouse PAR DNA occupies unusually long chromosome axes, potentially as shorter chromatin loops, predicted to promote double-strand break (DSB) formation. Most PARs show delayed appearance of RAD51/DMC1 foci, which mark DSB ends, and all PARs undergo delayed DSB-mediated homologous pairing. Analysis of Spo11β isoform-specific transgenic mice revealed that late RAD51/DMC1 foci in the PAR are genetically distinct from both early PAR foci and global foci and that late PAR foci promote efficient X-Y pairing, recombination, and male fertility. Our findings uncover specific mechanisms that surmount the unique challenges of X-Y recombination.

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