New Norfloxacin Resistance Gene in Pseudomonas Aeruginosa PAO

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1990 Sep 1

PMID 2126688

Citations 75

Authors

H Fukuda

M Hosaka

K Hirai

S Iyobe

Affiliations

Soon will be listed here.

Abstract

A new type of norfloxacin-resistant mutant of Pseudomonas aeruginosa PAO was isolated. This mutant showed cross resistance to imipenem and chloramphenicol and hypersusceptibility to beta-lactam and aminoglycoside antibiotics. The new norfloxacin resistance gene nfxC was mapped near catA (46 min) on the PAO chromosome. Norfloxacin accumulation was decreased in the nfxC mutant; furthermore, the rate of imipenem diffusion through the outer membrane of the nfxC mutant was lower than that of the parent strain. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane proteins showed a decrease of a 46-kilodalton protein and an increase of a 50-kilodalton protein in the nfxC mutant. We conclude the nfxC is a new norfloxacin resistance gene that affects outer membrane permeability to quinolones and other antimicrobial agents.

Citing Articles

Evolutionary loss of an antibiotic efflux pump increases quorum sensing mediated virulence .

Fernandes S, Ortega H, Vaillancourt M, Galdino A, Stotland A, Mun K Res Sq. 2024; .

PMID: 39606469 PMC: 11601840. DOI: 10.21203/rs.3.rs-5391023/v1.

Inhibition of quorum sensing by chemical induction of the MexEF-oprN efflux pump.

Kristensen R, Andersen J, Rybtke M, Jansen C, Fritz B, Kiilerich R Antimicrob Agents Chemother. 2024; 68(2):e0138723.

PMID: 38189278 PMC: 10848761. DOI: 10.1128/aac.01387-23.

Adaptation of biofilms to tobramycin and the quorum sensing inhibitor C-30 during experimental evolution requires multiple genotypic and phenotypic changes.

Bove M, Kolpen M, Lichtenberg M, Bjarnsholt T, Coenye T Microbiology (Reading). 2023; 169(1).

PMID: 36748633 PMC: 9993117. DOI: 10.1099/mic.0.001278.

and Antibiotic Efflux Pump Variants Exhibit Increased Virulence.

Vaillancourt M, Limsuwannarot S, Bresee C, Poopalarajah R, Jorth P Antibiotics (Basel). 2021; 10(10).

PMID: 34680745 PMC: 8532662. DOI: 10.3390/antibiotics10101164.

Reconstructing a Wild-Type Pseudomonas aeruginosa Reference Strain PAO1.

Lee S, Gallagher L, Manoil C J Bacteriol. 2021; 203(14):e0017921.

PMID: 33972355 PMC: 8223932. DOI: 10.1128/JB.00179-21.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Fujimaki K, Fujii T, Aoyama H, Sato K, Inoue Y, Inoue M . Quinolone resistance in clinical isolates of Serratia marcescens. Antimicrob Agents Chemother. 1989; 33(5):785-7. PMC: 172536. DOI: 10.1128/AAC.33.5.785. View

Hancock R, Nikaido H . Outer membranes of gram-negative bacteria. XIX. Isolation from Pseudomonas aeruginosa PAO1 and use in reconstitution and definition of the permeability barrier. J Bacteriol. 1978; 136(1):381-90. PMC: 218670. DOI: 10.1128/jb.136.1.381-390.1978. View

Matsumoto H, Ohta S, Kobayashi R, Terawaki Y . Chromosomal location of genes participating in the degradation of purines in Pseudomonas aeruginosa. Mol Gen Genet. 1978; 167(2):165-76. DOI: 10.1007/BF00266910. View

Hancock R, Carey A . Outer membrane of Pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins. J Bacteriol. 1979; 140(3):902-10. PMC: 216732. DOI: 10.1128/jb.140.3.902-910.1979. View

Matsumoto H, Nakazawa T, Ohta S, Terawaki Y . Chromosomal locations of catA, pobA, dcu and chu genes in Pseudomonas aeruginosa. Genet Res. 1981; 38(3):251-66. DOI: 10.1017/s0016672300020590. View

Okii M, Iyobe S, Mitsuhashi S . Mapping of the gene specifying aminoglycoside 3'-phosphotransferase II on the Pseudomonas aeruginosa chromosome. J Bacteriol. 1983; 155(2):643-9. PMC: 217734. DOI: 10.1128/jb.155.2.643-649.1983. View

Gutmann L, Williamson R, Moreau N, Kitzis M, Collatz E, Acar J . Cross-resistance to nalidixic acid, trimethoprim, and chloramphenicol associated with alterations in outer membrane proteins of Klebsiella, Enterobacter, and Serratia. J Infect Dis. 1985; 151(3):501-7. DOI: 10.1093/infdis/151.3.501. View

Yoshimura F, Nikaido H . Diffusion of beta-lactam antibiotics through the porin channels of Escherichia coli K-12. Antimicrob Agents Chemother. 1985; 27(1):84-92. PMC: 176210. DOI: 10.1128/AAC.27.1.84. View

10.

Rella M, Haas D . Resistance of Pseudomonas aeruginosa PAO to nalidixic acid and low levels of beta-lactam antibiotics: mapping of chromosomal genes. Antimicrob Agents Chemother. 1982; 22(2):242-9. PMC: 183719. DOI: 10.1128/AAC.22.2.242. View

11.

Sanders C, Watanakunakorn C . Emergence of resistance to beta-lactams, aminoglycosides, and quinolones during combination therapy for infection due to Serratia marcescens. J Infect Dis. 1986; 153(3):617-9. DOI: 10.1093/infdis/153.3.617. View

12.

Hooper D, Wolfson J, Souza K, Tung C, McHugh G, SWARTZ M . Genetic and biochemical characterization of norfloxacin resistance in Escherichia coli. Antimicrob Agents Chemother. 1986; 29(4):639-44. PMC: 180458. DOI: 10.1128/AAC.29.4.639. View

13.

Quinn J, Dudek E, DiVincenzo C, Lucks D, Lerner S . Emergence of resistance to imipenem during therapy for Pseudomonas aeruginosa infections. J Infect Dis. 1986; 154(2):289-94. DOI: 10.1093/infdis/154.2.289. View

14.

Hirai K, Aoyama H, SUZUE S, IRIKURA T, Iyobe S, Mitsuhashi S . Isolation and characterization of norfloxacin-resistant mutants of Escherichia coli K-12. Antimicrob Agents Chemother. 1986; 30(2):248-53. PMC: 180528. DOI: 10.1128/AAC.30.2.248. View

15.

Sato K, Inoue Y, Fujii T, Aoyama H, Inoue M, Mitsuhashi S . Purification and properties of DNA gyrase from a fluoroquinolone-resistant strain of Escherichia coli. Antimicrob Agents Chemother. 1986; 30(5):777-80. PMC: 176532. DOI: 10.1128/AAC.30.5.777. View

16.

Inoue Y, Sato K, Fujii T, Hirai K, Inoue M, Iyobe S . Some properties of subunits of DNA gyrase from Pseudomonas aeruginosa PAO1 and its nalidixic acid-resistant mutant. J Bacteriol. 1987; 169(5):2322-5. PMC: 212172. DOI: 10.1128/jb.169.5.2322-2325.1987. View

17.

Hirai K, SUZUE S, IRIKURA T, Iyobe S, Mitsuhashi S . Mutations producing resistance to norfloxacin in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1987; 31(4):582-6. PMC: 174781. DOI: 10.1128/AAC.31.4.582. View

18.

BUSCHER K, Cullmann W, Dick W, Opferkuch W . Imipenem resistance in Pseudomonas aeruginosa resulting from diminished expression of an outer membrane protein. Antimicrob Agents Chemother. 1987; 31(5):703-8. PMC: 174818. DOI: 10.1128/AAC.31.5.703. View

19.

Aoyama H, Sato K, Kato T, Hirai K, Mitsuhashi S . Norfloxacin resistance in a clinical isolate of Escherichia coli. Antimicrob Agents Chemother. 1987; 31(10):1640-1. PMC: 175005. DOI: 10.1128/AAC.31.10.1640. View

20.

Auckenthaler R, Regamey P, Pechere J . Resistance occurring after fluoroquinolone therapy of experimental Pseudomonas aeruginosa peritonitis. Antimicrob Agents Chemother. 1987; 31(11):1803-8. PMC: 175043. DOI: 10.1128/AAC.31.11.1803. View