Outer Membrane of Pseudomonas Aeruginosa: Heat- 2-mercaptoethanol-modifiable Proteins

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1979 Dec 1

PMID 118160

Citations 223

Authors

R E Hancock

A M Carey

Affiliations

Soon will be listed here.

Abstract

A number of polyacrylamide gel systems and solubilization procedures were studied to define the number and nature of "major" polypeptide bands in the outer membrane of Pseudomonas aeruginosa. It was shown that five of the eight major outer membrane proteins were "heat modifiable" in that their mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was determined by the solubilization temperature. Four of these heat-modifiable proteins had characteristics similar to protein II of the Escherichia coli outer membrane. Addition of lipopolysaccharide subsequent to solubilization caused reversal of the heat modification. The other heat-modifiable protein, the porin protein F, was unusually stable to sodium dodecyl sulfate. Long periods of boiling in sodium dodecyl sulfate were required to cause conversion to the heat-modified form. This was demonstrated both with outer membrane-associated and purified lipopolysaccharide-depleted protein F. Furthermore, lipopolysaccharide treatment had no effect on the mobility of heat-modified protein F. Thus it is concluded that protein F represents a new class of heat-modifiable protein. It was further demonstrated that the electrophoretic mobility of protein F was modified by 2-mercaptoethanol and that the 2-mercaptoethanol and heat modification of mobility were independent of one another. The optimal conditions for the examination of the outer membrane proteins of P. aeruginosa by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis are discussed.

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References

Hancock R, Nikaido H . Outer membranes of gram-negative bacteria. XIX. Isolation from Pseudomonas aeruginosa PAO1 and use in reconstitution and definition of the permeability barrier. J Bacteriol. 1978; 136(1):381-90. PMC: 218670. DOI: 10.1128/jb.136.1.381-390.1978. View

Mizuno T, Kageyama M . Isolation of characterization of a major outer membrane protein of Pseudomonas aeruginosa. Evidence for the occurrence of a lipoprotein. J Biochem. 1979; 85(1):115-22. DOI: 10.1093/oxfordjournals.jbchem.a132300. View

Uemura J, Mizushima S . Isolation of outer membrane proteins of Escherchia coli and their characterization on polyacrylamide gel. Biochim Biophys Acta. 1975; 413(2):163-76. DOI: 10.1016/0005-2736(75)90101-7. View

Garten W, Hindennach I, Henning U . The major proteins of the Escherichia coli outer cell envelope membrane. Characterization of proteins II* and III, comparison of all proteins. Eur J Biochem. 1975; 59(1):215-21. DOI: 10.1111/j.1432-1033.1975.tb02444.x. View

Hancock R, Decad G, Nikaido H . Identification of the protein producing transmembrane diffusion pores in the outer membrane of Pseudomonas aeruginosa PA01. Biochim Biophys Acta. 1979; 554(2):323-31. DOI: 10.1016/0005-2736(79)90373-0. View

Russell R . Two-dimensional SDS-polyacrylamide gel electrophoresis of heat-modifiable outer-membrane proteins. Can J Microbiol. 1976; 22(1):83-91. DOI: 10.1139/m76-011. View

Demartini M, Inouye M . Interaction between two major outer membrane proteins of Escherichia coli: the matrix protein and the lipoprotein. J Bacteriol. 1978; 133(1):329-35. PMC: 222011. DOI: 10.1128/jb.133.1.329-335.1978. View

Schweizer M, Hindennach I, Garten W, Henning U . Major proteins of the Escherichia coli outer cell envelope membrane. Interaction of protein II with lipopolysaccharide. Eur J Biochem. 1978; 82(1):211-7. DOI: 10.1111/j.1432-1033.1978.tb12013.x. View

DiRienzo J, Nakamura K, Inouye M . The outer membrane proteins of Gram-negative bacteria: biosynthesis, assembly, and functions. Annu Rev Biochem. 1978; 47:481-532. DOI: 10.1146/annurev.bi.47.070178.002405. View

10.

Henning U, Sonntag I, Hindennach I . Mutants (ompA) affecting a major outer membrane protein of Escherichia coli K12. Eur J Biochem. 1978; 92(2):491-8. DOI: 10.1111/j.1432-1033.1978.tb12771.x. View

11.

Booth B, Curtis N . Separation of the cytoplasmic and outer membrane of Pseudomonas aeruginosa PAQ. Biochem Biophys Res Commun. 1977; 74(3):1168-76. DOI: 10.1016/0006-291x(77)91641-2. View

12.

Stinson M, Cohen M, MERRICK J . Purification and properties of the periplasmic glucose-binding protein of Pseudomonas aeruginosa. J Bacteriol. 1977; 131(2):672-81. PMC: 235478. DOI: 10.1128/jb.131.2.672-681.1977. View

13.

Jarrell K, Kropinski A . Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO. J Virol. 1977; 23(3):461-6. PMC: 515855. DOI: 10.1128/JVI.23.3.461-466.1977. View

14.

McMichael J, Ou J . Metal ion dependence of a heat-modifiable protein from the outer membrane of Escherichia coli upon sodium dodecyl sulfate-gel electrophoresis. J Bacteriol. 1977; 132(1):314-20. PMC: 221858. DOI: 10.1128/jb.132.1.314-320.1977. View

15.

Matsushita K, Adachi O, Shinagawa E, Ameyama M . Isolation and characterization of outer and inner membranes from Pseudomonas aeruginosa and effect of EDTA on the membranes. J Biochem. 1978; 83(1):171-81. DOI: 10.1093/oxfordjournals.jbchem.a131888. View

16.

Stinnett J, Eagon R . Outer (cell wall) membrane proteins of Pseudomonas aeruginosa. Can J Microbiol. 1973; 19(12):1469-71. DOI: 10.1139/m73-239. View

17.

Schnaitman C . Outer membrane proteins of Escherichia coli. 3. Evidence that the major protein of Escherichia coli O111 outer membrane consists of four distinct polypeptide species. J Bacteriol. 1974; 118(2):442-53. PMC: 246776. DOI: 10.1128/jb.118.2.442-453.1974. View

18.

Osborn M, Gander J, Parisi E, Carson J . Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972; 247(12):3962-72. View

19.

Bragg P, Hou C . Organization of proteins in the native and reformed outer membrane of Escherichia coli. Biochim Biophys Acta. 1972; 274(2):478-88. DOI: 10.1016/0005-2736(72)90193-9. View

20.

Inouye M, Yee M . Homogeneity of envelope proteins of Escherichia coli separated by gel electrophoresis in sodium dodecyl sulfate. J Bacteriol. 1973; 113(1):304-12. PMC: 251632. DOI: 10.1128/jb.113.1.304-312.1973. View