Cysteine Cathepsins in Human Carious Dentin

Overview

Journal J Dent Res

Specialty Dentistry

Date 2011 Jan 21

PMID 21248362

Citations 59

Authors

F D Nascimento

C L Minciotti

S Geraldeli

M R Carrilho

D H Pashley

F R Tay

H B Nader

T Salo

L Tjaderhane

I L S Tersariol

Affiliations

Soon will be listed here.

Abstract

Matrix metalloproteinases (MMPs) are important in dentinal caries, and analysis of recent data demonstrates the presence of other collagen-degrading enzymes, cysteine cathepsins, in human dentin. This study aimed to examine the presence, source, and activity of cysteine cathepsins in human caries. Cathepsin B was detected with immunostaining. Saliva and dentin cysteine cathepsin and MMP activities on caries lesions were analyzed spectrofluorometrically. Immunostaining demonstrated stronger cathepsins B in carious than in healthy dentin. In carious dentin, cysteine cathepsin activity increased with increasing depth and age in chronic lesions, but decreased with age in active lesions. MMP activity decreased with age in both active and chronic lesions. Salivary MMP activities were higher in patients with active than chronic lesions and with increasing lesion depth, while cysteine cathepsin activities showed no differences. The results indicate that, along with MMPs, cysteine cathepsins are important, especially in active and deep caries.

Citing Articles

The activity, distribution, and colocalization of cathepsin K and matrix metalloproteases in intact and eroded dentin.

Lin X, Tong X, Chen Y, Huang Z, Yu H Clin Oral Investig. 2023; 28(1):1.

PMID: 38114764 DOI: 10.1007/s00784-023-05393-5.

Presence of host and bacterial-derived collagenolytic proteases in carious dentin: a systematic review of ex vivo studies.

Barbosa C, Monici Silva I, Cena J, Stefani C, Dame-Teixeira N Front Cell Infect Microbiol. 2023; 13:1278754.

PMID: 38029242 PMC: 10644316. DOI: 10.3389/fcimb.2023.1278754.

Dentin Degradation: From Tissue Breakdown to Possibilities for Therapeutic Intervention.

Vidal C, Carrilho M Curr Oral Health Rep. 2023; 10(3):99-110.

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The Role of Bacterial, Dentinal, Salivary, and Neutrophil Degradative Activity in Caries Pathogenesis.

Peled Y, Stewart C, Glogauer M, Finer Y Dent J (Basel). 2023; 11(9).

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Cathepsins in oral diseases: mechanisms and therapeutic implications.

Jiang H, Dong Z, Xia X, Li X Front Immunol. 2023; 14:1203071.

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