A Dynamic Mechanical Method to Assess Bulk Viscoelastic Behavior of the Dentin Extracellular Matrix

Overview

Journal Dent Mater

Specialty Dentistry

Date 2020 Nov 1

PMID 33129510

Citations 8

Authors

Yvette Alania

Mariana Cavalcante Dos Reis

Joo-Won Nam

Rasika S Phansalkar

James McAlpine

Shao-Nong Chen

Guido F Pauli

Ana K Bedran-Russo

Affiliations

Soon will be listed here.

Abstract

Objectives: To develop a protocol for assessment of the bulk viscoelastic behavior of dentin extracellular matrix (ECM), and to assess relationships between induced collagen cross-linking and viscoelasticity of the dentin ECM.

Methods: Dentin ECM was treated with agents to induce exogenous collagen cross-linking: proanthocyanidins (PACs) from Vitis vinifera - VVe, PACs from Pinus massoniana - PMe, glutaraldehyde - (GA), or kept untreated (control). A dynamic mechanical strain sweep method was carried out in a 3-point bending submersion clamp at treatment; after protein destabilization with 4 M urea and after 7-day, 6-month, and 12-month incubation in simulated body fluid. Tan δ, storage (E'), loss (E"), and complex moduli (E*) were calculated and data were statistically analyzed using two-way ANOVA and post-hoc tests (α = 0.05). Chemical analysis of dentin ECM before and after protein destabilization was assessed with ATR-FTIR spectroscopy.

Results: Significant interactions between study factors (treatment vs. time points, p < 0.001) were found for all viscoelastic parameters. Despite a significant decrease in all moduli after destabilization, PAC-treated dentin remained statistically higher than control (p < 0.001), indicating permanent mechanical enhancement after biomodification. Covalently crosslinked, GA-treated dentin was unaffected by destabilization (p = 0.873) and showed the lowest damping capacity (tan δ) at all time points (p < 0.001). After 12 months, the damping capacity of PMe and VVe groups decreased significantly. Changes in all amide IR resonances revealed a partial chemical reversal of PAC-mediated biomodification.

Significance: Viscoelastic measurements and IR spectroscopy aid in elucidating the role of inter-molecular collagen cross-linking in the mechanical behavior of dentin ECM.

Citing Articles

Preparation, Modification, Quantitation, and Dentin Biomodification Activity of Selectively Enriched Proanthocyanidins.

Jing S, Neves J, Liberato W, Ferreira D, Bedran-Russo A, McAlpine J J Nat Prod. 2025; 88(1):152-161.

PMID: 39804821 PMC: 11759650. DOI: 10.1021/acs.jnatprod.4c01213.

Prodelphinidins enhance dentin matrix properties and promote adhesion to methacrylate resin.

Reis-Havlat M, Leme-Kraus A, Alania Y, Zhou B, Tang Y, McAlpine J Dent Mater. 2024; 40(8):1164-1170.

PMID: 38871526 PMC: 11260231. DOI: 10.1016/j.dental.2024.05.024.

Modulatory role of terminal monomeric flavan-3-ol units in the viscoelasticity of dentin.

Reis-Havlat M, Alania Y, Zhou B, Jing S, McAlpine J, Chen S J Biomed Mater Res B Appl Biomater. 2023; 112(1):e35333.

PMID: 37792302 PMC: 10842555. DOI: 10.1002/jbm.b.35333.

B-Type Oligomers from Expand the Procyanidin Chemical Space and Exhibit Dental Bioactivity.

Zhou B, Alania Y, Reis M, Jing S, McAlpine J, Bedran-Russo A J Nat Prod. 2022; 85(12):2753-2768.

PMID: 36382951 PMC: 9789173. DOI: 10.1021/acs.jnatprod.2c00664.

B-type Proanthocyanidins with Dentin Biomodification Activity from Cocoa ().

Jing S, Reis M, Alania Y, McAlpine J, Chen S, Bedran-Russo A J Agric Food Chem. 2022; 70(39):12456-12468.

PMID: 36134876 PMC: 9547875. DOI: 10.1021/acs.jafc.2c04288.

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