A Kinase-independent Role for Unoccupied Insulin and IGF-1 Receptors in the Control of Apoptosis

Overview

Journal Sci Signal

Specialties Cell Biology
Physiology
Science

Date 2010 Dec 9

PMID 21139139

Citations 39

Authors

Jeremie Boucher

Yazmin Macotela

Olivier Bezy

Marcelo A Mori

Kristina Kriauciunas

C Ronald Kahn

Affiliations

Soon will be listed here.

Abstract

Insulin and insulin-like growth factor 1 (IGF-1) act as antiapoptotic hormones. We found that, unexpectedly, double-knockout (DKO) cells that lacked both insulin and IGF-1 receptors (IR and IGF1R, respectively) were resistant to apoptosis induced through either the intrinsic or the extrinsic pathway. This resistance to apoptosis was associated with decreased abundance of the proapoptotic protein Bax and increases in abundance of the antiapoptotic proteins Bcl-2, Bcl-xL, XIAP, and Flip. These changes in protein abundance involved primarily posttranscriptional mechanisms. Restoration of IR or IGF1R to DKO cells also restored their sensitivity to apoptosis. Notably, expression of a catalytically inactive mutant form of the IR also restored susceptibility to apoptosis. Thus, IR and IGF1R have bidirectional roles in the control of cell survival and can be viewed as dependence receptors. Insulin and IGF-1 binding stimulates receptor tyrosine kinase activity and blocks apoptosis, whereas unliganded IR and IGF1R, acting through a mechanism independent of their catalytic activity, exert a permissive effect on cell death.

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