Direct Reprogramming of Fibroblasts into Epiblast Stem Cells

Overview

Journal Nat Cell Biol

Specialty Cell Biology

Date 2010 Dec 7

PMID 21131959

Citations 60

Authors

Dong Wook Han

Boris Greber

Guangming Wu

Natalia Tapia

Marcos J Arauzo-Bravo

Kinarm Ko

Christof Bernemann

Martin Stehling

Hans R Scholer

Affiliations

Soon will be listed here.

Abstract

Epiblast stem cells (EpiSCs) derived from epiblast tissue of post-implantation embryos are pluripotent and can give rise to all three germ layers in teratoma assays. Introduction of the four transcription factors Oct4, Sox2, Klf4 and c-Myc into somatic cells has been shown to generate induced pluripotent stem cells (iPSCs) that are very similar to embryonic stem cells (ESCs) in a number of characteristics. However, generation of EpiSCs by the direct reprogramming of somatic cells using these transcription factors has not been shown to date. Here, we show that these transcription factors can be used to directly generate induced EpiSCs (iEpiSCs) under EpiSC culture conditions. iEpiSCs resemble EpiSCs in morphology, gene expression pattern, epigenetic status and chimaera-forming capability. This study demonstrates that the culture environment in transcription factor-mediated reprogramming determines the cell fate of the reprogrammed cell. We therefore hypothesize that it will eventually be possible to shape the identity of a directly reprogrammed cell simply by modulating culture conditions.

Citing Articles

The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells.

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The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells.

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Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions.

Pieri N, de Souza A, Botigelli R, Pessoa L, Recchia K, Machado L Stem Cell Rev Rep. 2021; 18(5):1639-1656.

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Down-regulation of Beclin1 promotes direct cardiac reprogramming.

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