Generation of Integration-free Induced Neural Stem Cells from Mouse Fibroblasts

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2016 May 19

PMID 27189941

Citations 13

Authors

Sung Min Kim

Jong-Wan Kim

Tae Hwan Kwak

Sang Woong Park

Kee-Pyo Kim

Hyunji Park

Kyung Tae Lim

Kyuree Kang

Jonghun Kim

Ji Hun Yang

Heonjong Han

Insuk Lee

Jung Keun Hyun

Young Min Bae

Hans R Scholer

Hoon Taek Lee

Dong Wook Han

Affiliations

Soon will be listed here.

Abstract

The viral vector-mediated overexpression of the defined transcription factors, Brn4/Pou3f4, Sox2, Klf4, and c-Myc (BSKM), could induce the direct conversion of somatic fibroblasts into induced neural stem cells (iNSCs). However, viral vectors may be randomly integrated into the host genome thereby increasing the risk for undesired genotoxicity, mutagenesis, and tumor formation. Here we describe the generation of integration-free iNSCs from mouse fibroblasts by non-viral episomal vectors containing BSKM. The episomal vector-derived iNSCs (e-iNSCs) closely resemble control NSCs, and iNSCs generated by retrovirus (r-iNSCs) in morphology, gene expression profile, epigenetic status, and self-renewal capacity. The e-iNSCs are functionally mature, as they could differentiate into all the neuronal cell types both in vitro and in vivo Our study provides a novel concept for generating functional iNSCs using a non-viral, non-integrating, plasmid-based system that could facilitate their biomedical applicability.

Citing Articles

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Generation of Porcine Induced Neural Stem Cells Using the Sendai Virus.

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