Thioredoxin-like 2 Regulates Human Cancer Cell Growth and Metastasis Via Redox Homeostasis and NF-κB Signaling

Overview

Journal J Clin Invest

Specialty General Medicine

Date 2010 Dec 3

PMID 21123948

Citations 63

Authors

Ying Qu

Jinhua Wang

Partha S Ray

Hua Guo

Jian Huang

Miyung Shin-Sim

Bolanle A Bukoye

Bingya Liu

Adrian V Lee

Xin Lin

Peng Huang

John W Martens

Armando E Giuliano

Ning Zhang

Ning-Hui Cheng

Xiaojiang Cui

Affiliations

Soon will be listed here.

Abstract

Cancer cells have an efficient antioxidant system to counteract their increased generation of ROS. However, whether this ability to survive high levels of ROS has an important role in the growth and metastasis of tumors is not well understood. Here, we demonstrate that the redox protein thioredoxin-like 2 (TXNL2) regulates the growth and metastasis of human breast cancer cells through a redox signaling mechanism. TXNL2 was found to be overexpressed in human cancers, including breast cancers. Knockdown of TXNL2 in human breast cancer cell lines increased ROS levels and reduced NF-κB activity, resulting in inhibition of in vitro proliferation, survival, and invasion. In addition, TXNL2 knockdown inhibited tumorigenesis and metastasis of these cells upon transplantation into immunodeficient mice. Furthermore, analysis of primary breast cancer samples demonstrated that enhanced TXNL2 expression correlated with metastasis to the lung and brain and with decreased overall patient survival. Our studies provided insight into redox-based mechanisms underlying tumor growth and metastasis and suggest that TXNL2 could be a target for treatment of breast cancer.

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