Progressive Resistance Voluntary Wheel Running in the Mdx Mouse

Overview

Journal Muscle Nerve

Date 2010 Nov 25

PMID 21104862

Citations 47

Authors

Jarrod A Call

James N McKeehen

Susan A Novotny

Dawn A Lowe

Affiliations

Soon will be listed here.

Abstract

Exercise training has been minimally explored as a therapy to mitigate the loss of muscle strength for individuals with Duchenne muscular dystrophy (DMD). Voluntary wheel running is known to elicit beneficial adaptations in the mdx mouse model for DMD. The aim of this study was to examine progressive resistance wheel running in mdx mice by comprehensively testing muscle function before, during, and after a 12-week training period. Male mdx mice at ~4 weeks age were randomized into three groups: Sedentary, Free Wheel, and Resist Wheel. Muscle strength was assessed via in vivo dorsiflexion torque, grip strength, and whole body tension intermittently throughout the training period. Contractility of isolated soleus muscles was analyzed at the study's conclusion. Both Free and Resist Wheel mice had greater grip strength (~22%) and soleus muscle specific tetanic force (26%) compared with Sedentary mice. This study demonstrates that two modalities of voluntary exercise are beneficial to dystrophic muscle and may help establish parameters for an exercise prescription for DMD.

Citing Articles

Conditional ablation in the skeletal muscle and brain causes profound effects on muscle function, neurobehavior, and extracellular matrix pathways.

Karuppasamy M, English K, Conner J, Rorrer S, Lopez M, Crossman D bioRxiv. 2025; .

PMID: 39975305 PMC: 11838426. DOI: 10.1101/2025.01.30.635777.

Mitochondrial antioxidant SkQ1 attenuates C26 cancer-induced muscle wasting in males and improves muscle contractility in female tumor-bearing mice.

Tsitkanou S, Morena da Silva F, Cabrera A, Schrems E, Muhyudin R, Koopmans P Am J Physiol Cell Physiol. 2024; 327(5):C1308-C1322.

PMID: 39344417 PMC: 11559642. DOI: 10.1152/ajpcell.00497.2024.

Immunological regulation of skeletal muscle adaptation to exercise.

Langston P, Mathis D Cell Metab. 2024; 36(6):1175-1183.

PMID: 38670108 PMC: 11153001. DOI: 10.1016/j.cmet.2024.04.001.

Stuart has got the PoWeR! Skeletal muscle adaptations to a novel heavy progressive weighted wheel running exercise model in C57BL/6 mice.

Koopmans P, Williams-Frey T, Zwetsloot K Exp Physiol. 2023; 109(2):271-282.

PMID: 37974360 PMC: 10988744. DOI: 10.1113/EP091494.

Females display relatively preserved muscle quality compared with males during the onset and early stages of C26-induced cancer cachexia.

Cabrera A, Deaver J, Lim S, Morena da Silva F, Schrems E, Saling L J Appl Physiol (1985). 2023; 135(3):655-672.

PMID: 37535708 PMC: 10642509. DOI: 10.1152/japplphysiol.00196.2023.

References

Pardo J, Siliciano J, Craig S . A vinculin-containing cortical lattice in skeletal muscle: transverse lattice elements ("costameres") mark sites of attachment between myofibrils and sarcolemma. Proc Natl Acad Sci U S A. 1983; 80(4):1008-12. PMC: 393517. DOI: 10.1073/pnas.80.4.1008. View

Hayes A, Williams D . Beneficial effects of voluntary wheel running on the properties of dystrophic mouse muscle. J Appl Physiol (1985). 1996; 80(2):670-9. DOI: 10.1152/jappl.1996.80.2.670. View

Vrbova G . Function induced modifications of gene expression: an alternative approach to gene therapy of Duchenne muscular dystrophy. J Muscle Res Cell Motil. 2004; 25(2):187-92. DOI: 10.1023/b:jure.0000035893.59267.47. View

Lynch G . Role of contraction-induced injury in the mechanisms of muscle damage in muscular dystrophy. Clin Exp Pharmacol Physiol. 2004; 31(8):557-61. DOI: 10.1111/j.1440-1681.2004.04026.x. View

Hodges B, Hayashi Y, Nonaka I, Wang W, Arahata K, Kaufman S . Altered expression of the alpha7beta1 integrin in human and murine muscular dystrophies. J Cell Sci. 1998; 110 ( Pt 22):2873-81. DOI: 10.1242/jcs.110.22.2873. View

Bloch R, Gonzalez-Serratos H . Lateral force transmission across costameres in skeletal muscle. Exerc Sport Sci Rev. 2003; 31(2):73-8. DOI: 10.1097/00003677-200304000-00004. View

Rybakova I, Patel J, Davies K, Yurchenco P, Ervasti J . Utrophin binds laterally along actin filaments and can couple costameric actin with sarcolemma when overexpressed in dystrophin-deficient muscle. Mol Biol Cell. 2002; 13(5):1512-21. PMC: 111123. DOI: 10.1091/mbc.01-09-0446. View

Lehti T, Kalliokoski R, Komulainen J . Repeated bout effect on the cytoskeletal proteins titin, desmin, and dystrophin in rat skeletal muscle. J Muscle Res Cell Motil. 2007; 28(1):39-47. DOI: 10.1007/s10974-007-9102-0. View

STREET S, RAMSEY R . Sarcolemma: transmitter of active tension in frog skeletal muscle. Science. 1965; 149(3690):1379-80. DOI: 10.1126/science.149.3690.1379. View

10.

Carlson C, Makiejus R . A noninvasive procedure to detect muscle weakness in the mdx mouse. Muscle Nerve. 1990; 13(6):480-4. DOI: 10.1002/mus.880130603. View

11.

Reed P, Bloch R . Postnatal changes in sarcolemmal organization in the mdx mouse. Neuromuscul Disord. 2005; 15(8):552-61. DOI: 10.1016/j.nmd.2005.03.007. View

12.

Landisch R, Kosir A, Nelson S, Baltgalvis K, Lowe D . Adaptive and nonadaptive responses to voluntary wheel running by mdx mice. Muscle Nerve. 2008; 38(4):1290-303. PMC: 3392332. DOI: 10.1002/mus.21141. View

13.

Dupont-Versteegden E, McCarter R, Katz M . Voluntary exercise decreases progression of muscular dystrophy in diaphragm of mdx mice. J Appl Physiol (1985). 1994; 77(4):1736-41. DOI: 10.1152/jappl.1994.77.4.1736. View

14.

Aitkens S, McCrory M, Kilmer D, BERNAUER E . Moderate resistance exercise program: its effect in slowly progressive neuromuscular disease. Arch Phys Med Rehabil. 1993; 74(7):711-5. DOI: 10.1016/0003-9993(93)90031-5. View

15.

Carter G, Wineinger M, Walsh S, Horasek S, Abresch R, FOWLER Jr W . Effect of voluntary wheel-running exercise on muscles of the mdx mouse. Neuromuscul Disord. 1995; 5(4):323-32. DOI: 10.1016/0960-8966(94)00063-f. View

16.

Warren G, Moran A, Hogan H, Lin A, Guldberg R, Lowe D . Voluntary run training but not estradiol deficiency alters the tibial bone-soleus muscle functional relationship in mice. Am J Physiol Regul Integr Comp Physiol. 2007; 293(5):R2015-26. DOI: 10.1152/ajpregu.00569.2007. View

17.

Lovering R, De Deyne P . Contractile function, sarcolemma integrity, and the loss of dystrophin after skeletal muscle eccentric contraction-induced injury. Am J Physiol Cell Physiol. 2003; 286(2):C230-8. PMC: 4489567. DOI: 10.1152/ajpcell.00199.2003. View

18.

Baltgalvis K, Call J, Nikas J, Lowe D . Effects of prednisolone on skeletal muscle contractility in mdx mice. Muscle Nerve. 2009; 40(3):443-54. PMC: 2879072. DOI: 10.1002/mus.21327. View

19.

Ervasti J, Campbell K . Membrane organization of the dystrophin-glycoprotein complex. Cell. 1991; 66(6):1121-31. DOI: 10.1016/0092-8674(91)90035-w. View

20.

Hanft L, Rybakova I, Patel J, Rafael-Fortney J, Ervasti J . Cytoplasmic gamma-actin contributes to a compensatory remodeling response in dystrophin-deficient muscle. Proc Natl Acad Sci U S A. 2006; 103(14):5385-90. PMC: 1459364. DOI: 10.1073/pnas.0600980103. View