Regulatory Effects of Ribosomal S6 Kinase 1 (RSK1) in IFNλ Signaling

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2010 Nov 16

PMID 21075852

Citations 15

Authors

Barbara Kroczynska

Sonali Joshi

Elizabeth A Eklund

Amit Verma

Sergei V Kotenko

Eleanor N Fish

Leonidas C Platanias

Affiliations

Soon will be listed here.

Abstract

Although the mechanisms of generation of signals that control transcriptional activation of Type III IFN (IFNλ)-regulated genes have been identified, very little is known about the mechanisms by which the IFNλ receptor generates signals for mRNA translation of IFNλ-activated genes. We provide evidence that IFNλ activates the p90 ribosomal protein S6 kinase 1 (RSK1) and its downstream effector, initiation factor eIF4B. Prior to its engagement by the IFNλ receptor, the non-active form of RSK1 is present in a complex with the translational repressor 4E-BP1 in IFNλ-sensitive cells. IFNλ-inducible phosphorylation/activation of RSK1 results in its dissociation from 4E-BP1 at the same time that 4E-BP1 dissociates from eIF4E to allow formation of eIF4F and initiation of cap-dependent translation. Our studies demonstrate that such IFNλ-dependent engagement of RSK1 is essential for up-regulation of p21(WAF1/CIP1) expression, suggesting a mechanism for generation of growth-inhibitory responses. Altogether, our data provide evidence for a critical role for the activated RSK1 in IFNλ signaling.

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