Expression of Two "immediate Early" Genes, Egr-1 and C-fos, in Response to Renal Ischemia and During Compensatory Renal Hypertrophy in Mice

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1990 Mar 1

PMID 2107209

Citations 28

Authors

A J Ouellette

R A Malt

V P Sukhatme

J V Bonventre

Affiliations

Soon will be listed here.

Abstract

To identify specific genetic regulatory mechanisms associated with renal ischemia, we measured the accumulation of Egr-1 and c-fos mRNAs in the mouse kidney after occlusion of the renal artery and reperfusion. At 1 h after right nephrectomy and arterial occlusion of the contralateral kidney for 10 or 30 min, Egr-1 mRNA levels were three to five times greater in these kidneys as compared with those in control animals that had sustained unilateral nephrectomy alone and were much greater than levels in the normal organ. Whether ischemia was imposed for 10 or for 30 min, renal Egr-1 mRNA contents were equivalent and remained elevated after 24 h of reperfusion subsequent to 30 min of ischemia. Although c-fos mRNA also accumulated in response to ischemia and reperfusion, the pattern differed from that of Egr-1 in that c-fos mRNA content varied with the duration of ischemia and was undetectable 24 h after injury. Contralateral nephrectomy was not necessary to see the marked accumulation of Egr-1 and c-fos mRNAs with unilateral ischemia. Reflow was necessary, however, since only minimal sequence accumulation occurred by the end of the ischemic period. After left uninephrectomy alone, Egr-1 mRNA levels in the remaining kidney were maximal 30 min after surgery, but were not detectable thereafter; c-fos mRNA levels did not change after unilateral nephrectomy. Differential expression of early growth-related genes implicated in transcriptional activation may influence tissue recovery after renal ischemia.

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References

Arendshorst W, FINN W, GOTTSCHALK C . Pathogenesis of acute renal failure following temporary renal ischemia in the rat. Circ Res. 1975; 37(5):558-68. DOI: 10.1161/01.res.37.5.558. View

Buscher M, Rahmsdorf H, Litfin M, Karin M, Herrlich P . Activation of the c-fos gene by UV and phorbol ester: different signal transduction pathways converge to the same enhancer element. Oncogene. 1988; 3(3):301-11. View

Venkatachalam M, Bernard D, Donohoe J, LEVINSKY N . Ischemic damage and repair in the rat proximal tubule: differences among the S1, S2, and S3 segments. Kidney Int. 1978; 14(1):31-49. DOI: 10.1038/ki.1978.87. View

Rave N, Crkvenjakov R, Boedtker H . Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels. Nucleic Acids Res. 1979; 6(11):3559-67. PMC: 327956. DOI: 10.1093/nar/6.11.3559. View

Halliburton I, THOMSON R . Chemical aspects of compensatory renal hypertrophy. Cancer Res. 1965; 25(11):1882-7. View

Malt R, LeMaitre D . Accretion and turnover of RNA in the renoprival kidney. Am J Physiol. 1968; 214(5):1041-7. DOI: 10.1152/ajplegacy.1968.214.5.1041. View

Malt R, Lemaittre D . Nucleic acids in fetal kidney after maternal nephrectomy. Proc Soc Exp Biol Med. 1969; 130(2):539-42. DOI: 10.3181/00379727-130-35600. View

PERRY R, La Torre J, Kelley D, Greenberg J . On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. Biochim Biophys Acta. 1972; 262(2):220-6. DOI: 10.1016/0005-2787(72)90236-5. View

Reimer K, GANOTE C, Jennings R . Alterations in renal cortex following ischemic injury. 3. Ultrastructure of proximal tubules after ischemia or autolysis. Lab Invest. 1972; 26(4):347-63. View

10.

FINN W, Chevalier R . Recovery from postischemic acute renal failure in the rat. Kidney Int. 1979; 16(2):113-23. DOI: 10.1038/ki.1979.112. View

11.

Chirgwin J, Przybyla A, MacDonald R, Rutter W . Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979; 18(24):5294-9. DOI: 10.1021/bi00591a005. View

12.

Muller R, Slamon D, TREMBLAY J, Cline M, Verma I . Differential expression of cellular oncogenes during pre- and postnatal development of the mouse. Nature. 1982; 299(5884):640-4. DOI: 10.1038/299640a0. View

13.

Ouellette A, WATSON R, Billmire K, Dygert M, Ingwall J . Protein synthesis in the cultured fetal mouse heart: effects of deprivation of oxygen and oxidizable substrate. Biochemistry. 1983; 22(5):1201-7. DOI: 10.1021/bi00274a033. View

14.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

15.

Kelly K, Cochran B, Stiles C, Leder P . Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell. 1983; 35(3 Pt 2):603-10. DOI: 10.1016/0092-8674(83)90092-2. View

16.

Toback F . Phosphatidylcholine metabolism during renal growth and regeneration. Am J Physiol. 1984; 246(3 Pt 2):F249-59. DOI: 10.1152/ajprenal.1984.246.3.F249. View

17.

Feinberg A, Vogelstein B . "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984; 137(1):266-7. DOI: 10.1016/0003-2697(84)90381-6. View

18.

Greenberg M, Ziff E . Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature. 1984; 311(5985):433-8. DOI: 10.1038/311433a0. View

19.

Goyette M, Petropoulos C, Shank P, Fausto N . Regulated transcription of c-Ki-ras and c-myc during compensatory growth of rat liver. Mol Cell Biol. 1984; 4(8):1493-8. PMC: 368939. DOI: 10.1128/mcb.4.8.1493-1498.1984. View

20.

Wu B, Morimoto R . Transcription of the human hsp70 gene is induced by serum stimulation. Proc Natl Acad Sci U S A. 1985; 82(18):6070-4. PMC: 390701. DOI: 10.1073/pnas.82.18.6070. View