Evaluation of Three Commercially Available Japanese Encephalitis Virus IgM Enzyme-linked Immunosorbent Assays

Overview

Journal Am J Trop Med Hyg

Specialty Tropical Medicine

Date 2010 Nov 2

PMID 21036854

Citations 24

Authors

Jaimie S Robinson

David Featherstone

Ravi Vasanthapuram

Brad J Biggerstaff

Anita Desai

Nalini Ramamurty

Anwarul Haque Chowdhury

Hardeep S Sandhu

Kathleen F Cavallaro

Barbara W Johnson

Affiliations

Soon will be listed here.

Abstract

We evaluated performance of three commercial Japanese encephalitis virus (JEV) IgM antibody capture enzyme-linked immunosorbent assay (MAC ELISA) kits with a panel of serological specimens collected during a surveillance project of acute encephalitis syndrome in India and acute meningitis and encephalitis syndrome in Bangladesh. The serum and cerebral spinal fluid specimens had been referred to the Centers for Disease Control and Prevention (CDC) for confirmatory testing. The CDC results and specimen classifications were considered the reference standard. All three commercial kits had high specificity (95-99.5%), but low sensitivities, ranging from 17-57%, with both serum and cerebrospinal fluid samples. Specific factors contributing to low sensitivity compared with the CDC ELISA could not be determined through further analysis of the limits and dilution end points of IgM detection.

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