High-cell-density Cyclic Fed-batch Fermentation of a Poly(3-hydroxybutyrate)-accumulating Thermophile, Chelatococcus Sp. Strain MW10

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2010 Oct 5

PMID 20889784

Citations 18

Authors

Mohammad H A Ibrahim

Alexander Steinbuchel

Affiliations

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Abstract

Different fermentation strategies were employed for the cultivation of a new poly(3-hydroxybutyrate)-accumulating thermophilic bacterium, Chelatococcus sp. strain MW10, with the aim of achieving high-cell-density (HCD) growth and high poly(3-hydroxybutyrate) [poly(3HB)] productivity. Enhanced cultivation was achieved by a cyclic fed-batch fermentation (CFBF) technique (42-liter scale). Maximal poly(3HB) productivity was obtained during the second cycle [16.8 ± 4.2 g poly(3HB)/liter]. At the end of CFBF (265 h), an HCD of up to 115.0 ± 4.3 g cell dry weight/liter was achieved.

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