Characterization of Newly Isolated Thermotolerant Bacterium Cupriavidus Sp. CB15 from Composting and Its Ability to Produce Polyhydroxyalkanoate from Glycerol

Overview

Journal Microb Cell Fact

Publisher Biomed Central

Specialties Biotechnology
Microbiology

Date 2023 Apr 12

PMID 37046250

Authors

Anuyut Yootoum

Kittisak Jantanasakulwong

Pornchai Rachtanapun

Churairat Moukamnerd

Thanongsak Chaiyaso

Chayakorn Pumas

Nuttapol Tanadchangsaeng

Masanori Watanabe

Toshiaki Fukui

Chayatip Insomphun

Affiliations

Soon will be listed here.

Abstract

Background: This study aimed to isolate a novel thermotolerant bacterium that is capable of synthesizing polyhydroxyalkanoate from glycerol under high temperature conditions.

Results: A newly thermotolerant polyhydroxyalkanoate (PHA) producing bacterium, Cupriavidus sp. strain CB15, was isolated from corncob compost. The potential ability to synthesize PHA was confirmed by detection of PHA synthase (phaC) gene in the genome. This strain could produce poly(3-hydroxybutyrate) [P(3HB)] with 0.95 g/L (PHA content 75.3 wt% of dry cell weight 1.24 g/L) using glycerol as a carbon source. The concentration of PHA was enhanced and optimized based on one-factor-at-a-time (OFAT) experiments and response surface methodology (RSM). The optimum conditions for growth and PHA biosynthesis were 10 g/L glycerol, 0.78 g/L NHCl, shaking speed at 175 rpm, temperature at 45 °C, and cultivation time at 72 h. Under the optimized conditions, PHA production was enhanced to 2.09 g/L (PHA content of 74.4 wt% and dry cell weight of 2.81 g/L), which is 2.12-fold compared with non-optimized conditions. Nuclear magnetic resonance (NMR) analysis confirmed that the extracted PHA was a homopolyester of 3-hydyoxybutyrate.

Conclusion: Cupriavidus sp. strain CB15 exhibited potential for cost-effective production of PHA from glycerol.

Citing Articles

High-Yield Production of Polyhydroxybutyrate and Poly(3-hydroxybutyrate--3-hydroxyhexanoate) from Crude Glycerol by a Newly Isolated Species Oh_219.

Oh S, Lim G, Han Y, Kim W, Joo H, Kim Y Polymers (Basel). 2025; 17(2).

PMID: 39861268 PMC: 11768116. DOI: 10.3390/polym17020197.

Microbial Biosynthesis of Medium-Chain-Length Polyhydroxyalkanoate (mcl-PHA) from Waste Cooking Oil.

Elazzazy A, Ali Abd K, Bataweel N, Mahmoud M, Baghdadi A Polymers (Basel). 2024; 16(15).

PMID: 39125176 PMC: 11314287. DOI: 10.3390/polym16152150.

Advances in stress-tolerance elements for microbial cell factories.

Kuang Z, Yan X, Yuan Y, Wang R, Zhu H, Wang Y Synth Syst Biotechnol. 2024; 9(4):793-808.

PMID: 39072145 PMC: 11277822. DOI: 10.1016/j.synbio.2024.06.008.

Paracoccus kondratievae produces poly(3-hydroxybutyrate) under elevated temperature conditions.

Moanis R, Geeraert H, Van den Brande N, Hennecke U, Peeters E Environ Microbiol Rep. 2024; 16(3):e13260.

PMID: 38838099 PMC: 11150862. DOI: 10.1111/1758-2229.13260.

Recent Challenges and Trends of Polyhydroxyalkanoate Production by Extremophilic Bacteria Using Renewable Feedstocks.

Mozejko-Ciesielska J, Ray S, Sankhyan S Polymers (Basel). 2023; 15(22).

PMID: 38006109 PMC: 10674690. DOI: 10.3390/polym15224385.

References

Thompson D, Ovenden T, Pennycott T, Nager R . The prevalence and source of plastic incorporated into nests of five seabird species on a small offshore island. Mar Pollut Bull. 2020; 154:111076. DOI: 10.1016/j.marpolbul.2020.111076. View

Zahari M, Ariffin H, Mokhtar M, Salihon J, Shirai Y, Hassan M . Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238(T)). J Biomed Biotechnol. 2012; 2012:125865. PMC: 3477595. DOI: 10.1155/2012/125865. View

Liu Y, Huang S, Zhang Y, Xu F . Isolation and characterization of a thermophilic Bacillus shackletonii K5 from a biotrickling filter for the production of polyhydroxybutyrate. J Environ Sci (China). 2014; 26(7):1453-62. DOI: 10.1016/j.jes.2014.05.011. View

Pernicova I, Novackova I, Sedlacek P, Kourilova X, Koller M, Obruca S . Application of osmotic challenge for enrichment of microbial consortia in polyhydroxyalkanoates producing thermophilic and thermotolerant bacteria and their subsequent isolation. Int J Biol Macromol. 2019; 144:698-704. DOI: 10.1016/j.ijbiomac.2019.12.128. View

Singh M, Kumar P, Patel S, Kalia V . Production of Polyhydroxyalkanoate Co-polymer by Bacillus thuringiensis. Indian J Microbiol. 2014; 53(1):77-83. PMC: 3587498. DOI: 10.1007/s12088-012-0294-7. View

Musilova J, Kourilova X, Pernicova I, Bezdicek M, Lengerova M, Obruca S . Novel thermophilic polyhydroxyalkanoates producing strain Aneurinibacillus thermoaerophilus CCM 8960. Appl Microbiol Biotechnol. 2022; 106(12):4669-4681. DOI: 10.1007/s00253-022-12039-1. View

An J, Ha B, Kuk Lee S . Production of polyhydroxyalkanoates by the thermophile Cupriavidus cauae PHS1. Bioresour Technol. 2023; 371:128627. DOI: 10.1016/j.biortech.2023.128627. View

Pagliano G, Ventorino V, Panico A, Pepe O . Integrated systems for biopolymers and bioenergy production from organic waste and by-products: a review of microbial processes. Biotechnol Biofuels. 2017; 10:113. PMC: 5414342. DOI: 10.1186/s13068-017-0802-4. View

Zahari M, Zakaria M, Ariffin H, Mokhtar M, Salihon J, Shirai Y . Renewable sugars from oil palm frond juice as an alternative novel fermentation feedstock for value-added products. Bioresour Technol. 2012; 110:566-71. DOI: 10.1016/j.biortech.2012.01.119. View

10.

Estrada-de Los Santos P, Solano-Rodriguez R, Matsumura-Paz L, Vasquez-Murrieta M, Martinez-Aguilar L . Cupriavidus plantarum sp. nov., a plant-associated species. Arch Microbiol. 2014; 196(11):811-7. DOI: 10.1007/s00203-014-1018-7. View

11.

Kourilova X, Pernicova I, Vidlakova M, Krejcirik R, Mrazova K, Hrubanova K . Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium . Bioengineering (Basel). 2021; 8(10). PMC: 8533406. DOI: 10.3390/bioengineering8100141. View

12.

Wei Y, Chen W, Huang C, Wu H, Sun Y, Lo C . Screening and evaluation of polyhydroxybutyrate-producing strains from indigenous isolate Cupriavidus taiwanensis strains. Int J Mol Sci. 2011; 12(1):252-65. PMC: 3039951. DOI: 10.3390/ijms12010252. View

13.

Choonut A, Prasertsan P, Klomklao S, Sangkharak K . Bacillus thermoamylovorans-Related Strain Isolated from High Temperature Sites as Potential Producers of Medium-Chain-Length Polyhydroxyalkanoate (mcl-PHA). Curr Microbiol. 2020; 77(10):3044-3056. DOI: 10.1007/s00284-020-02118-9. View

14.

Mozejko-Ciesielska J, Kiewisz R . Bacterial polyhydroxyalkanoates: Still fabulous?. Microbiol Res. 2016; 192:271-282. DOI: 10.1016/j.micres.2016.07.010. View

15.

Xu Z, Pan C, Li X, Hao N, Zhang T, Gaffrey M . Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440. Biotechnol Biofuels. 2021; 14(1):11. PMC: 7792162. DOI: 10.1186/s13068-020-01861-2. View

16.

Satoh Y, Tajima K, Nakamoto S, Xuerong H, Matsushima T, Ohshima T . Isolation of a thermotolerant bacterium producing medium-chain-length polyhydroxyalkanoate. J Appl Microbiol. 2011; 111(4):811-7. DOI: 10.1111/j.1365-2672.2011.05093.x. View

17.

Bhatia S, Gurav R, Choi T, Jung H, Yang S, Moon Y . Bioconversion of plant biomass hydrolysate into bioplastic (polyhydroxyalkanoates) using Ralstonia eutropha 5119. Bioresour Technol. 2018; 271:306-315. DOI: 10.1016/j.biortech.2018.09.122. View

18.

KOMINEK L, HALVORSON H . Metabolism of poly-beta-hydroxybutyrate and acetoin in Bacillus cereus. J Bacteriol. 1965; 90(5):1251-9. PMC: 315809. DOI: 10.1128/jb.90.5.1251-1259.1965. View

19.

Ibrahim M, Steinbuchel A . High-cell-density cyclic fed-batch fermentation of a poly(3-hydroxybutyrate)-accumulating thermophile, Chelatococcus sp. strain MW10. Appl Environ Microbiol. 2010; 76(23):7890-5. PMC: 2988602. DOI: 10.1128/AEM.01488-10. View

20.

Gahlawat G, Soni S . Valorization of waste glycerol for the production of poly (3-hydroxybutyrate) and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer by Cupriavidus necator and extraction in a sustainable manner. Bioresour Technol. 2017; 243:492-501. DOI: 10.1016/j.biortech.2017.06.139. View