Characterization of Zebrafish Intestinal Smooth Muscle Development Using a Novel Sm22α-b Promoter

Overview

Journal Dev Dyn

Publisher Wiley

Specialty Anatomy & Histology

Date 2010 Oct 1

PMID 20882680

Citations 23

Authors

Christoph Seiler

Joshua Abrams

Michael Pack

Affiliations

Soon will be listed here.

Abstract

Smooth muscle cells provide structural support for many tissues and control essential physiological processes, such as blood pressure and gastrointestinal motility. Relatively little is known about the early stages of intestinal smooth muscle development and its relationship to the development of the enteric nervous system, which regulates intestinal motility. Here, we report an evolutionarily conserved 523 base pair regulatory element within the promoter of the zebrafish sm22α-b (transgelin1) gene that directs transgene expression in smooth muscle cells of the intestine and other tissues. Comparative genomic analysis identified a conserved motif within this element consisting of two Serum Response Factor binding sites that is also present in the promoters of many mammalian smooth muscle genes. We established a stable line expressing GFP in smooth muscle cell and used this line to describe lineage relationships among cells within different intestinal smooth muscle layers and their co-development with the enteric nervous system (ENS).

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References

Pietsch J, Delalande J, Jakaitis B, Stensby J, Dohle S, Talbot W . lessen encodes a zebrafish trap100 required for enteric nervous system development. Development. 2006; 133(3):395-406. PMC: 2651469. DOI: 10.1242/dev.02215. View

Kuhlman J, Eisen J . Genetic screen for mutations affecting development and function of the enteric nervous system. Dev Dyn. 2006; 236(1):118-27. DOI: 10.1002/dvdy.21033. View

Hendrix J, Wamhoff B, McDonald O, Sinha S, Yoshida T, Owens G . 5' CArG degeneracy in smooth muscle alpha-actin is required for injury-induced gene suppression in vivo. J Clin Invest. 2005; 115(2):418-27. PMC: 546420. DOI: 10.1172/JCI22648. View

Muller F, Blader P, Strahle U . Search for enhancers: teleost models in comparative genomic and transgenic analysis of cis regulatory elements. Bioessays. 2002; 24(6):564-72. DOI: 10.1002/bies.10096. View

Kelsh R, Eisen J . The zebrafish colourless gene regulates development of non-ectomesenchymal neural crest derivatives. Development. 2000; 127(3):515-25. DOI: 10.1242/dev.127.3.515. View

Holmberg A, Olsson C, Holmgren S . The effects of endogenous and exogenous nitric oxide on gut motility in zebrafish Danio rerio embryos and larvae. J Exp Biol. 2006; 209(Pt 13):2472-9. DOI: 10.1242/jeb.02272. View

Olden T, Akhtar T, Beckman S, Wallace K . Differentiation of the zebrafish enteric nervous system and intestinal smooth muscle. Genesis. 2008; 46(9):484-98. DOI: 10.1002/dvg.20429. View

Osbourn J, Weissberg P, Shanahan C . A regulatory element downstream of the rat SM22 alpha gene transcription start point enhances reporter gene expression in vascular smooth muscle cells. Gene. 1995; 154(2):249-53. DOI: 10.1016/0378-1119(94)00847-l. View

Villefranc J, Amigo J, Lawson N . Gateway compatible vectors for analysis of gene function in the zebrafish. Dev Dyn. 2007; 236(11):3077-87. PMC: 4518551. DOI: 10.1002/dvdy.21354. View

10.

Wang D, Olson E . Control of smooth muscle development by the myocardin family of transcriptional coactivators. Curr Opin Genet Dev. 2004; 14(5):558-66. DOI: 10.1016/j.gde.2004.08.003. View

11.

Kawakami K, Shima A . Identification of the Tol2 transposase of the medaka fish Oryzias latipes that catalyzes excision of a nonautonomous Tol2 element in zebrafish Danio rerio. Gene. 1999; 240(1):239-44. DOI: 10.1016/s0378-1119(99)00444-8. View

12.

Yang X, Yao J, Cheng L, Wei D, Xue J, Lu D . Molecular cloning and expression of a smooth muscle-specific gene SM22alpha in zebrafish. Biochem Biophys Res Commun. 2003; 312(3):741-6. DOI: 10.1016/j.bbrc.2003.10.185. View

13.

Peri F, Nusslein-Volhard C . Live imaging of neuronal degradation by microglia reveals a role for v0-ATPase a1 in phagosomal fusion in vivo. Cell. 2008; 133(5):916-27. DOI: 10.1016/j.cell.2008.04.037. View

14.

Kawakami K, Takeda H, Kawakami N, Kobayashi M, Matsuda N, Mishina M . A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Dev Cell. 2004; 7(1):133-44. DOI: 10.1016/j.devcel.2004.06.005. View

15.

Teg Pipes G, Creemers E, Olson E . The myocardin family of transcriptional coactivators: versatile regulators of cell growth, migration, and myogenesis. Genes Dev. 2006; 20(12):1545-56. DOI: 10.1101/gad.1428006. View

16.

Holmberg A, Schwerte T, Pelster B, Holmgren S . Ontogeny of the gut motility control system in zebrafish Danio rerio embryos and larvae. J Exp Biol. 2004; 207(Pt 23):4085-94. DOI: 10.1242/jeb.01260. View

17.

Assinder S, Stanton J, Prasad P . Transgelin: an actin-binding protein and tumour suppressor. Int J Biochem Cell Biol. 2008; 41(3):482-6. DOI: 10.1016/j.biocel.2008.02.011. View

18.

Dutton K, Pauliny A, Lopes S, Elworthy S, Carney T, Rauch J . Zebrafish colourless encodes sox10 and specifies non-ectomesenchymal neural crest fates. Development. 2001; 128(21):4113-25. DOI: 10.1242/dev.128.21.4113. View

19.

Xu R, Ho Y, Ritchie R, Li L . Human SM22 alpha BAC encompasses regulatory sequences for expression in vascular and visceral smooth muscles at fetal and adult stages. Am J Physiol Heart Circ Physiol. 2003; 284(4):H1398-407. DOI: 10.1152/ajpheart.00737.2002. View

20.

Ovcharenko I, Loots G, Giardine B, Hou M, Ma J, Hardison R . Mulan: multiple-sequence local alignment and visualization for studying function and evolution. Genome Res. 2004; 15(1):184-94. PMC: 540288. DOI: 10.1101/gr.3007205. View